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期刊论文
High-level expression of a synthetic gene encoding a sweet protein, monellin, in Escherichia coli
Biotechnology Letters (2005) 27: 1745-1749，-0001，（）：
The expression of a synthetic gene encoding monellin, a sweet protein, in E. coli under the control of T7 promoter from phage is described. The single-chain monellin gene was designed based on the biased codons of E. coli so as to optimize its expression. Monellin was produced and accounted for 45% of total soluble proteins. It was purified to yield 43 mg protein per g dry cell wt. The purity of the recombinant protein was confirmed by SDS-PAGE.
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