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期刊论文
The construction of dicistron system to improve the expression level of bFGF in Eseherichia eoli*
中国病理生理杂志,2003,19(5):627~631,-0001,():
AIM: To investigate the condition necessay for high-level expression of basic fibrob-last growth factor (hbFGF) in Escherichia coli, adicistron system was constructed. METHODS:1he phage T7 gene φ10 N-tenninal sequence, present in the expression plasmid pET-3c, was incolporated into the first cistron, the coding region for bFCF was incorporated into the second, and boLh were under the control of a transcnption promotor recogruzed by the bacteriophage T7 RNA polymerase. RESULTS: Recambinants were transfonned into BI21(DE3) and induced by IPTG for expression, accumulation of up to 20% of the total ceU protein of bFGF were obtained, showing bioactivity indistingushable from standard protein. CONCLUSION: It suggested that such dicistron system was effective for enhancing hbFCF expression.
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