The cotyledons of Xinjiang cantaloupes were transfonned with strain of Agrobaarium tumefaciens LBA4404, harboring expresaon vector pBIC-35sNPl. By selection of regenerated plantlets with Kanamycin resistance and PCR assay, putative transgenic plantlets were bebeved to being obtained. In this research. the influence factors of cantaloupe transformation were discussed, the preferable protocol is 3 days cotyledon culture and re-suspended bacterium. OD600 0.6, with liquid MS before transformation.lnoculation and co-culture duration were 8～12min, 3d.And KmlOOmg/L, Amp 500mfl/L supplied to development media (MS+ BA l.Omg/L+IBA 0.2mg/L) is proper. During transfonnation, 0.2mg/L man- nitol treatment is effective (effiency increased 6.6%) but As is not so. Ihis transformation metIlod could be routinely used for the introduction of