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期刊论文
枯草芽孢杆菌(Bacillus subtilis)S9对植物病原真菌的溶菌作用
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Nine hundred and seventy-six bacterial isolates were obtained in this study, among which a bacterial antagonistic isolate, Bacillus subtilis S9 from sugarcane rhizosphere showed cell-lytic effect on the plant pathogens, Rhizoctonia solani, Pythium ultimum and Fusarium oxysporum f. sp. niveum after 4-day-confronting incubation on PDA plates. However, S9 could not cause obvious inhibitory zones on PDA plates during this incubation. Lytic spots of hyphae of R. solani caused by S9 were clearly presented under scanning electronic microscope. The cell-lytic process of S9 is as follows: Bacterial cells of S9 absorb on hypae of R. solani firstly, and grow and reproduce as the fungal hyphae grow, and then secrete some cell-lytic substances to decompose the hyphae. However, this study revealed that S9 hardly interfered with the growth of the fungal antagonists, such as Trichoderma viride, Chaetomium cupreum and Chaetomium globosum. Pot experiments proved that S9 could effectively control root disease of tomato caused by R. solani. It Suggested that S9 mixed with above fungal antagonists could potentially synergistically control plant diseases caused by pathogenic fungi.
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