A mature appressorium cDNA library of rice blast fungus, Magnaporthe grisea, was constructed in a λ TriplEx2 vector by SMARTTM cDNA library containing 2.37×10.6 independent clones about 100% of which harbor foreign cDNA inserts with average size of 660bp. Of 9 randomly selected clones, 2 expressed tags (ESTs) sequences did not have homologous EST sequences of M. grisea in GenBank. The appressorium cDNA library is suitable for gene expression analysis and function analysis of the late stages of appressorium formation and the carly stages of penctration of M. grisea.