Selecting by indirect ELISA eleven hybridoma cell lines, secreting monoclonal antibodies (McAbs) against Magnaporthe grisea were produced by fusing mouse myeloma cells (SP2/0) with spleen cells from BALB/c mice immunized with the mixture of conidia、germ tubes and appressoria of M. grisea using 50% PEG. The result of IFTC test showed that four McAbs, named as 2B4、4A1、1D1、and 2H4 specificlly bound to the cell wall surface of the fungus; Western blotting revealed that 2B4、4A1、1D1 were recognized different protein antigens from the surface of conidia and germ tubes; These four McAbs could effectively interfere with the appressorium formation both on cellophane membrance and the surface of onion epidermis and inhibit the disease leaf lesions development in vitro test.