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期刊论文
Sparc (Osteonectin) functions in morphogenesis of the pharyngealskeleton and inner ear
Matrix Biology 27(2008)561-572,-0001,():
Sparc (Osteonectin), a matricellular glycoprotein expressed by many differentiated cells, is a major non-collagenous constituent of vertebratebones. Recent studies indicate that Sparc expression appears early in development, although its function and regulation during embryogenesis arelargely unknown. We cloned zebrafish sparc and investigated its role during development, using a mo rpholino antisense oligonucleotide-basedknockdown approach. Consistent with its strong expression in the otic vesicle and developing pharyngeal cartilages, knockdown of Sparc functionresulted in specific inner ear and cartilage defects that are highlighted by changes in gene expression, morphology and behavior. We rescued theknockdown phenotypes by co-injecting sparc mRNA, providing evidence that the knockdown phenotype is due specifically to impairment ofSparc function. A comparison of the phenotypes of Sparc knockdown and known zebrafish mutants with similar defects places Sparc downstreamof sox9 in the genetic network that regulates development of the pharyngeal skeleton and inner ear of vertebrates.
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