The function and usage of vMIPα encoded by K6 gene of herpesvirus 8 (HHV8) which has homology with human macrophage protein (MIP) have not been clearly known. In the present note the K6 gene of HHV8 was cloned and transfected into NIH3T3 cells and E. coli cells. Condi-tional media from the 3T3-transfected cells and K6 product vMIPα from E. coli. Cells were used to perform the experi-ments of ligand-receptor binding and cellular adhesion with peripheral blood macrophages. The conditional media and the purified vMIPα from E. coli could compete to bind to CCR5 located on macrophages from peripheral blood with 125 I-hMIP-1α chemokine of human. Cellular adhesion showed that the conditional media from transfected cells and the purified vMIPα did not induce the adhesion of mac-rophages from peripheral blood to ICAM-1. In conclusion, vMIPα encoded by K6 gene of HHV8 can bind to CCR5 of peripheral blood macrophage cells and does not induce their adhesion. This suggests that vMIPα enclosed CCR5, also known as HIV co-receptor, may be used to prevent and treat HIV infection.