-
29浏览
-
0点赞
-
0收藏
-
0分享
-
142下载
-
0评论
-
引用
期刊论文
Saikosaponin a inhibits the proliferation and activation of T cells through cell cycle arrest and induction of apoptosis
International Immunopharmacology 9 (2009) 978-983,-0001,():
In the present study, we aimed at examining the immunosuppressive activity of saikosaponin a, a triterpene saponin derived from Bupleurum falcatum L. (Umbelliferae), and the underlying mechanisms. Saikosaponin a significantly inhibited the proliferation and activation of T cells activated by concanavalin A (Con A) in a concentration-dependent manner. Additionally, it potently suppressed Con A-stimulated IL-2, IFN-γ and TNF-α production inmouse Tcells. Saikosaponin a also causedG0/G1 arrest of activated Tcells through down-regulating the protein levels of CDK6 and Cyclin D3 and up-regulating the protein level of p27kip. Furthermore, the compound dose-dependently induced apoptosis of Con A-activated T cells rather than those non-activated, as determined by Annexin V/PI staining. Besides, it induced a remarkable collapse of mitochondrial membrane potential and caused significant release of cytochrome c from mitochondria to cytosol. In summary, these results suggest that the G0/G1 arrest aswell as the induction of apoptosis viamitochondrial pathway are involved in the immunosuppressive activity of saikosaponin a against activated T cells. This may herald a novel approach for further studies of saikosaponin a as a candidate for the treatment of inflammatory and autoimmune diseases.
【免责声明】以下全部内容由[孙洋]上传于[2010年09月25日 17时05分35秒],版权归原创者所有。本文仅代表作者本人观点,与本网站无关。本网站对文中陈述、观点判断保持中立,不对所包含内容的准确性、可靠性或完整性提供任何明示或暗示的保证。请读者仅作参考,并请自行承担全部责任。
本学者其他成果
同领域成果