Rice blast disease, caused by the fungalpathogen Pyricularia grisea Sacc., is one of the mostdevastating crop diseases worldwide. Previous studieshave shown that the dominant blast resistance genePi-2(t) confers resistance to a broad spectrum ofpathogenic strains. Using a population of 292 recombinantinbred lines combined with bioinformatic analysis,we mapped Pi-2(t) between the SSR (simple-sequencerepeat) marker SSR140 and the RFLP (restrictionfragment length polymorphism) marker JSH12, 0.9 cMfrom both SSR140 and JSH12. A physical map consistingof six overlapping BAC (bacterial artificialchromosome) clones was anchored to the region containingthe Pi-2(t) locus. By analyzing recombinationevents in this region, the Pi-2(t) locus was localized to aDNA fragment of 118 kb in length. The detailed geneticand physical maps of the Pi-2(t) locus will facilitateboth molecular isolation of the gene and marker-assistedtransfer of the gene in breeding programs.