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期刊论文
Purification, characterization and gene cloningof a novel phospholipase A2 from the venom ofAgkistrodon blomhoffii ussurensis
The International Journal of Biochemistry & Cell Biology 37(2005)558-565，-0001，（）：
A new phospholipase A2 with Gln at the site 49, abbreviated as Gln49-PLA2, has been purified from the venom of Agkistrodonblomhoffii ussurensis by using ion-exchange chromatography, gel filtration chromatography and reversed-phase HPLC, andbehaves as a single-band on SDS-PAGE. Its molecular weight is 13881.85 ± 0.33 Da given by mass spectrometry and pI is about8.56 given by isoelectric focusing. Gln49-PLA2 does not show phospholipase A2 and hemorrhagic activity, whereas shows weaktoxic and apparent anticoagulant activity. Based on the N-terminal sequencing and peptide mass fingerprint analysis, Gln49-PLA2cDNA has been cloned by means of RT-PCR. Gln49-PLA2 consists of 122 amino acid residues and has the structural featuresof class II of snake venom phospholipase A2.
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