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期刊论文

Genetic Transformation of Brassica campestris L. ssp. pekinensis via Agrobacterium- with Bt Gene

叶志彪Wang Feng Li Hanxia Ye Zhibiao* and Lu Yong’en

Hunan Agricultural Science & Technology Newsletter Vol.3 No.4,-0001,():

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摘要/描述

By means of Agrobacterium-mediated transformation, 43 kanamycin-resistant buds of Chinese cabbage were got. PCR, PCR-Southern blot and dot blot analysis were used to identify and characterize the putative transgenic plants. 26 plants had the predicted bands of the fragment of npt II gene. Insect bioassays of 4 transformants showed that toxic protein had been translated and the translation levels were different among these transformants.

【免责声明】以下全部内容由[叶志彪]上传于[2011年02月14日 08时36分09秒],版权归原创者所有。本文仅代表作者本人观点,与本网站无关。本网站对文中陈述、观点判断保持中立,不对所包含内容的准确性、可靠性或完整性提供任何明示或暗示的保证。请读者仅作参考,并请自行承担全部责任。

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