作物学报(英文版)
论文题目:Identification and fine mapping of two blast resistance genes in rice cultivar 93-11
全部作者: Cailin Leia,1, Kun Haoa,1, Yilong Yanga, Jian Maa, Shuai Wanga, Jiulin Wanga, Zhijun Chenga, Shasha Zhaoa, Xin Zhanga, Xiuping Guoa, Chunming Wangb, Jianmin Wana,b,
期刊名称:作物学报(英文版) 2013年第1期
论文摘要:Rice blast, caused by Magnaporthe oryzae, is a major disease of rice almost worldwide. The Chinese indica cultivar 93-11 is resistant to numerous isolates of the blast fungus in China, and can be used as broad-spectrum resistance resource, particularly in japonica rice breeding programs. In this...[查看全部]
论文摘要:Rice blast, caused by Magnaporthe oryzae, is a major disease of rice almost worldwide. The Chinese indica cultivar 93-11 is resistant to numerous isolates of the blast fungus in China, and can be used as broad-spectrum resistance resource, particularly in japonica rice breeding programs. In this study, we identified and mapped two blast resistance genes, Pi60(t) and Pi61(t), in cv. 93-11 using F2 and F3 populations derived from a cross between the susceptible cv. Lijiangxintuanheigu (LTH) and resistant cv. 93-11 and inoculated with M. oryzae isolates from different geographic origins. Pi60(t) was delimited to a 274 kb region on the short arm of chromosome 11, flanked by InDelmarkers K1-4 and E12 and cosegregatedwith InDel markers B1 and Y10. Pi61(t) was mapped to a 200 kb region on the short arm (near the centromere) of chromosome 12, flanked by InDel markers M2 and S29 and cosegregating with InDel marker M9. In the 274 kb region of Pi60(t), 93-11 contains six NBS-LRR genes including the two Pia/PiCO39 alleles (BGIOSGA034263 and BGIOSGA035032) which are quite close to the two Pia/ PiCO39 alleles (SasRGA4 and SasRGA5) in Sasanishiki and CO39, with only nine amino acids differing in the protein sequences of BGIOSGA035032 and SasRGA5. In the 200 kb region of Pi61(t), 93-11 contains four NBS-LRR genes, all of which show high identities in protein sequence with their corresponding NBS-LRR alleles in susceptible cv. Nipponbare. Comparison of the response spectra and physical positions between the target genes and other R genes in the same chromosome regions indicated that Pi60(t) could be Pia/PiCO39 or its allele, whereas Pi61(t) appears to be different from Pita, Pita-2, Pi19(t), Pi39(t) and Pi42(t) in the same R gene cluster. DNA markers tightly linked to Pi60(t) and Pi61(t) will enable marker-assisted breeding and map-based cloning.[返回]
下载频数:9 上传时间:2014-04-03
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论文题目:Zea mays (L.) P1 locus for cob glume color identified as a post-domestication selection target with an effect on temperate maize genomes
全部作者: Chuanxiao Xiea,1, Jianfeng Wenga,1, Wenguo Liuc, Cheng Zoua, Zhuanfang Haoa, Wenxue Lia, Minshun Lia, Xiaosen Guod, Gengyun Zhangd, Yunbi Xua,b, Xinhai Lia, Shihuang Zhanga,
期刊名称:作物学报(英文版) 2013年第1期
论文摘要:Artificial selection during domestication and post-domestication improvement results in loss of genetic diversity near target loci. However, the genetic locus associated with cob glume color and the nature of the genomic pattern surrounding it was elusive and the selection effect in that region...[查看全部]
下载频数:7 上传时间:2014-04-03
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论文题目:Genome-wide association of 10 horticultural traits with expressed sequence tag-derived SNP markers in a collection of lettuce lines
全部作者: Soonjae Kwona, Ivan Simkob, Barbara Helliera, Beiquan Moub, Jinguo Hua,
期刊名称:作物学报(英文版) 2013年第1期
论文摘要:Genetic diversity, population structure, and genome-wide marker-trait association analyses were conducted on a special collection of 298 homozygous lettuce (Lactuca sativa L.) lines. Each of these lines was derived from a single plant that had been genotyped with 384 SNP markers using LSGermOPA....[查看全部]
论文摘要:Genetic diversity, population structure, and genome-wide marker-trait association analyses were conducted on a special collection of 298 homozygous lettuce (Lactuca sativa L.) lines. Each of these lines was derived from a single plant that had been genotyped with 384 SNP markers using LSGermOPA. They included 122 butterhead, 53 romaine, 63 crisphead, 53 leaf and 7 stem types. Genetic diversity among these plants was assessed by pairwise comparison based on 322 high-quality SNP markers selected from 384 SNPs. Only 258 unique genotypes were identified among the 298 lines because 26 pairs or small groups (a total of 66 lines) shared identical genotypes. The average genetic similarity coefficient (GS) among these unique genotypes was 63.9% with a range of 40.6% to 99.8%. A phylogenetic tree was constructed based on the genotypic data. The most likely number of populations was estimated to be two or six. Association analysis between the 322 SNP markers and 10 phenotypic traits using the 258 homozygous lines was performed by three different methods: single factor analysis, general linear model analysis, and mixed linear model analysis. Nine significant marker-trait associations (SMTAs) were detected at P < 0.0001 with all three methods and also when considering kinship and/or population structure for this collection, with five SMTAs for seed coat color, one for leaf undulation, two for leaf anthocyanin, and one for stem anthocyanin. These markers will be useful in marker-assisted selection after further validation with segregating populations. Production and hosting by Elsevier B.V. on behalf of Crop Science Society of China and Institute of Crop Science, CAAS.[返回]
下载频数:6 上传时间:2014-04-03
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论文题目:Identification of unconditional and conditional QTL for oil, protein and starch content in maize
全部作者: Yuqiu Guoa,b, Xiaohong Yanga, Subhash Chandera, Jianbing Yana,c, Jun Zhanga, Tongming Songa, Jiansheng Lia,
期刊名称:作物学报(英文版) 2013年第1期
论文摘要:Oil, protein and starch are key chemical components of maize kernels. A population of 245 recombinant inbred lines (RILs) derived from a cross between a high-oil inbred line, By804, and a regular inbred line, B73, was used to dissect the genetic interrelationships among oil, starch and protein...[查看全部]
论文摘要:Oil, protein and starch are key chemical components of maize kernels. A population of 245 recombinant inbred lines (RILs) derived from a cross between a high-oil inbred line, By804, and a regular inbred line, B73, was used to dissect the genetic interrelationships among oil, starch and protein content at the individual QTL level by unconditional and conditional QTL mapping. Combined phenotypic data over two years with a genetic linkage map constructed using 236 markers, nine, five and eight unconditional QTL were detected for oil, protein and starch content, respectively. Some QTL for oil, protein and starch content were clustered in the same genomic regions and the direction of their effects was consistent with the sign of their correlation. In conditional QTL mapping, 37 (29/8) unconditional QTL were not detected or showed reduced effects, four QTL demonstrated similar effects under unconditional and conditional QTL mapping, and 17 additional QTL were identified by conditional QTL mapping. These results imply that there is a strong genetic relationship among oil, protein and starch content in maize kernels. The information generated in the present investigation could be helpful in marker-assisted breeding for maize varieties with desirable kernel quality traits.[返回]
下载频数:6 上传时间:2014-04-03
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论文题目:Anatomical and chemical characteristics associated with lodging resistance in wheat
全部作者: Eryan Konga, Dongcheng Liua, Xiaoli Guoc, Wenlong Yanga, Jiazhu Suna, Xin Lia, Kehui Zhanb, Dangqun Cuib, Jinxing Lind, Aimin Zhanga,b,
期刊名称:作物学报(英文版) 2013年第1期
论文摘要:Anatomical and chemical characteristics of stems affect lodging in wheat (Triticum aestivum L.) cultivars. Traits associated with lodging resistance, such as plant height, stem strength, culm wall thickness, pith diameter, and stem diameter, were extensively investigated in earlier studies....[查看全部]
论文摘要:Anatomical and chemical characteristics of stems affect lodging in wheat (Triticum aestivum L.) cultivars. Traits associated with lodging resistance, such as plant height, stem strength, culm wall thickness, pith diameter, and stem diameter, were extensively investigated in earlier studies. However, the solid stem trait was rarely considered. In this study, we measured a range of anatomical and chemical characteristics on solid and hollow stemmed wheat cultivars. Significant correlations were detected between resistance to lodging and several anatomical features, including width of mechanical tissue, weight of low internodes, and width of stem walls. Morphological features that gave the best indication of improved lodging resistance were increased stemwidth, width of mechanical tissue layer, and stem density. Multiple linear regression analysis showed that 99% of the variation in lodging resistance could be explained by the width of the mechanical tissue layer, suggesting that solid stemmed wheat has several anatomical features for increasing resistance to lodging. In addition, microsatellite markers GWM247 and GWM340 were linked to a single solid stem QTL on chromosome 3BL in a population derived from the cross Xinongshixin (solid stem)/Line 3159 (hollow stem). These markers should be valuable in breeding wheat for solid stem.[返回]
下载频数:7 上传时间:2014-04-03
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