作物学报(英文版)
论文题目:Induction of avirulence by AVR-Pita1 in virulent U.S. field isolates of Magnaporthe oryzae,
全部作者: Yuntao Daia,b, Eugenia Winstonc, James C. Corrella, Yulin Jiab,
期刊名称:作物学报(英文版) 2014年第1期
论文摘要:The AVR-Pita1 gene, from the Chinese isolate O-137 of Magnaporthe oryzae, is an effector that determines the efficacy of the Pi-ta rice blast resistance gene. In the present study, the avirulence function of AVR-Pita1 was induced by transformation of field isolates (TM2, ZN19, B2 and B8) that...[查看全部]
下载频数:13 上传时间:2014-04-03
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论文题目:Molecular characterization of α-gliadin genes from common wheat cultivar Zhengmai 004 and their role in quality and celiac disease
全部作者: Yuge Li, Ranran Xin, Dale Zhang, Suoping Li
期刊名称:作物学报(英文版) 2014年第1期
论文摘要:A total of 43 unique clones (Z4A-1 to Z4A-43 with GenBank accession numbers of HM120221, HM120222, JX828270, JN831402 to JN831406, and KC715889 to KC715923, respectively) were amplified and cloned from common wheat cultivar Zhengmai 004 using a PCR-based strategy. They included 22 full-ORF...[查看全部]
论文摘要:A total of 43 unique clones (Z4A-1 to Z4A-43 with GenBank accession numbers of HM120221, HM120222, JX828270, JN831402 to JN831406, and KC715889 to KC715923, respectively) were amplified and cloned from common wheat cultivar Zhengmai 004 using a PCR-based strategy. They included 22 full-ORF α-gliadin genes and 21 pseudogenes containing at least one in-framestop codon. Comparative analysis of the deduced amino acid sequences showed that all the isolated genes displayed the typical structural features of α-gliadin genes and that the putative proteins of Z4A-7, Z4A-14, Z4A-17 and Z4A-20 had an extra cysteine residue in the unique domain II, while Z4A-15 lacked the second conserved cysteine residue in the unique domain I. The two fusion proteins of Z4A-15 and Z4A-20 were successfully detected by SDS-PAGE and Western blotting, although the protein level was relatively low. Based on the occurrence of the fourmajor epitopes, aswell as the lengths of the two glutamine repeats, 8, 6, and 8 geneswere assigned to the Gli-2 loci on the respective chromosomes 6A, 6B, and 6Dand a total of respectively 16, 0 and 23 immunogenic peptides were identified. In addition, 4 of the 5 genes with odd numbers of cysteine residues were assigned to chromosome 6D, suggesting that common wheat cultivar Zhengmai 004 has the potential to induce celiac disease (CD) and that the D genome exerts themost influence on gluten quality, but is the most deleterious for CD patients. By phylogenetic analysis, 11 exceptional α-gliadins with few or no immunogenic peptides from Triticum monococcum and Aegilops tauschii were detected, a finding that further supports the prospect of CD prevention. Finally, secondary structure prediction showed that most (98.48%) of the α-gliadins invariably contained five conserved α-helices (H1 to H5) in the two glutamine repeats and unique domains and 67.68% of the α-gliadins also contained a β-strand (S) in the C-terminal unique domains. [返回]
下载频数:11 上传时间:2014-04-03
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论文题目:Near-infrared spectroscopy (NIRS) evaluation and regional analysis of Chinese faba bean (Vicia faba L.)
全部作者: Jiaojiao Wang1, Hao Liu1, Guixing Ren
期刊名称:作物学报(英文版) 2014年第1期
论文摘要:To analyze the nutritional composition of faba bean (Vicia faba L.) seed, estimation models were developed for protein, starch, oil, and total polyphenol using near infrared spectroscopy (NIRS). Two hundred and forty-four samples fromtwelve producing regionsweremeasured in both milled powder and...[查看全部]
论文摘要:To analyze the nutritional composition of faba bean (Vicia faba L.) seed, estimation models were developed for protein, starch, oil, and total polyphenol using near infrared spectroscopy (NIRS). Two hundred and forty-four samples fromtwelve producing regionsweremeasured in both milled powder and intact seed forms. Partial least squares (PLS) regression was applied for model development. The model based on ground seed powder was generally superior to that based on the intact seed. The optimal seed powder-basedmodels for protein, starch, and total polyphenol had coefficients of correlation (r2) of 0.97, 0.93 and 0.89, respectively. The relationship between nutrient contents and twelve producing areas was determined by two-step cluster analysis. Three distinct groupings were obtained with region-constituent features, i.e., Group 1 of high oil, Group 2 of high protein, and Group 3 of high starch as well as total polyphenol. The clustering accuracy was 79.5%. Moreover, the nutrition contents were affected by seeding date, longitude, latitude, and altitude of plant location. Cluster analysis revealed that the differences in the seed were strongly influenced by geographical factors.[返回]
下载频数:10 上传时间:2014-04-03
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论文题目:Induced defense responses in rice plants against small brown planthopper infestation
全部作者: Canxing Duan, Jiaojiao Yu, Jianyu Bai, Zhendong Zhu, Xiaoming Wang
期刊名称:作物学报(英文版) 2014年第1期
论文摘要:The small brown planthopper (SBPH), Laodelphax striatellus Fallén (Homoptera: Delphacidae), is a serious pest of rice (Oryza sativa L.) in China. To understand the mechanisms of rice resistance to SBPH, defense response genes and related defense enzymes were examined in resistant and susceptible...[查看全部]
论文摘要:The small brown planthopper (SBPH), Laodelphax striatellus Fallén (Homoptera: Delphacidae), is a serious pest of rice (Oryza sativa L.) in China. To understand the mechanisms of rice resistance to SBPH, defense response genes and related defense enzymes were examined in resistant and susceptible rice varieties in response to SBPH infestation. The salicylic acid (SA) synthesis-related genes phenylalanine ammonia-lyase (PAL), NPR1, EDS1 and PAD4 were induced rapidly and to a much higher level in the resistant variety Kasalath than in the susceptible cultivarWuyujing 3 in response to SBPH infestation. The expression level of PAL in the Kasalath rice at 12 h post-infestation (hpi) increased 7.52-fold compared with the un-infested control, and the expression level in Kasalath was 49.63, 87.18, 57.36 and 75.06 times greater than that in Wuyujing 3 at 24, 36, 48 and 72 hpi, respectively. However, the transcriptional levels of the jasmonic acid (JA) synthesis-related genes LOX and AOS2 in resistant Kasalath were significantly lower than in susceptible Wuyujing 3 at 24, 36, 48 and 72 hpi. The activities of the defense enzymes PAL, peroxidase (POD), and polyphenol oxidase (PPO) increased remarkably in Kasalath in response to SBPH infestation, and were closely correlated with the PAL gene transcript level. Our results indicated that the SA signaling pathway was activated in the resistant Kasalath rice variety in response to SBPH infestation and that the gene PAL played a considerable role in the resistance to SBPH.[返回]
下载频数:20 上传时间:2014-04-03
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论文题目:Impacts of nighttime post-anthesis warming on rice productivity and grain quality in East China
全部作者: Wenjun Donga,b, Jin Chenb, Lili Wangb, Yunlu Tianb, Bin Zhangb, Yongcai Laia, Ying Menga, Chunrong Qiana, Jia Guoc
期刊名称:作物学报(英文版) 2014年第1期
论文摘要:The impacts of nighttime post-anthesis warming on rice productivity and grain quality in East China were evaluated for two cultivars, II You 128, an indica rice, and Wuyunjing 7, a japonica rice. Warming by 3.0 °C stimulated the nighttime respiration rate and decreased the photosynthesis rate,...[查看全部]
下载频数:13 上传时间:2014-04-03
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论文题目:Integration of QTL detection and marker assisted selection for improving resistance to Fusarium head blight and important agronomic traits in wheat
全部作者: Chao Lva,b, Yanxia Songb, Lifeng Gaob, Qin Yaob, Ronghua Zhoub, Rugen Xua, Jizeng Jiab,
期刊名称:作物学报(英文版) 2014年第1期
论文摘要:Fusarium head blight (FHB), caused by Fusarium graminearum, is one of the most destructive wheat (Triticum aestivum L.) diseases worldwide. Identification of quantitative trait loci (QTL) conferring FHB resistance followed bymarker assisted selection (MAS) is an efficient approach to breed...[查看全部]
下载频数:11 上传时间:2014-04-03
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