作物学报(英文版)
论文题目:Genome-wide analysis of the WRKY transcription factor gene family in Gossypium raimondii and the expression of orthologs in cultivated tetraploid cotton
全部作者: Caiping Cai,Erli Niu,Hao Du,Liang Zhao,Yue Feng,Wangzhen GuO
期刊名称:作物学报(英文版) 2014年第2,3期
论文摘要:WRKY proteins are members of a family of transcription factors in higher plants that function in plant responses to various physiological processes. We identified 120 candidate WRKY genes from Gossypium raimondii with corresponding expressed sequence tags in at least one of four cotton...[查看全部]
论文摘要:WRKY proteins are members of a family of transcription factors in higher plants that function in plant responses to various physiological processes. We identified 120 candidate WRKY genes from Gossypium raimondii with corresponding expressed sequence tags in at least one of four cotton species,Gossypium hirsutum,Gossypium barbadense,Gossypium arboreum,and G. raimondii. These WRKY members were anchored on 13 chromosomes in G. raimondii with uneven distribution. Phylogenetic analysis showed that WRKY candidate genes can be classified into three groups,with 20 members in group I,88 in group II,and 12 in group III. The 88 genes in group II were further classified into five subgroups,groups IIa-e,containing 7,16,37,15,and 13 members,respectively. We characterized diversity in amino acid residues in the WRKY domain and/or other zinc finger motif regions in the WRKY proteins. The expression patterns of WRKY genes revealed their important roles in diverse functions in cotton developmental stages of vegetative and reproductive growth and stress response. Structural and expression analyses show that WRKY proteins are a class of important regulators of growth and development and play key roles in response to stresses in cotton.[返回]
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论文题目:Differential microRNA expression between shoots and rhizomes in Oryza longistaminata using high-throughput RNA sequencing
全部作者: Ying Zonga,Liyu Huanga,Ting Zhanga,Qiao Qina,Wensheng Wanga,Xiuqin Zhac,Fengyi HUb,Binying Fua,* Zhikang Lia
期刊名称:作物学报(英文版) 2014年第2,3期
论文摘要:Plant microRNAs (miRNAs) play important roles in biological processes such as developmentand stress responses. Although the diverse functions of miRNAs in model organisms have beenwell studied,their function in wild rice is poorly understood. In this study,high-throughputsmall RNA sequencing was...[查看全部]
论文摘要:Plant microRNAs (miRNAs) play important roles in biological processes such as developmentand stress responses. Although the diverse functions of miRNAs in model organisms have beenwell studied,their function in wild rice is poorly understood. In this study,high-throughputsmall RNA sequencing was performed to characterize tissue-specific transcriptomes in Oryzalongistaminata. A total of 603 miRNAs,380 known rice miRNAs,72 conserved plant miRNAs,and151 predicted novel miRNAs were identified as being expressed in aerial shoots and rhizomes.Additionally,99 and 79 miRNAs were expressed exclusively or differentially,respectively,in thetwo tissues,and 144 potential targets were predicted for the differentially expressed miRNAs inthe rhizomes. Functional annotation of these targets suggested that transcription factors,including squamosa promoter binding proteins and auxin response factors,function in rhizomegrowth and development. The expression levels of several miRNAs and target genes in therhizomes were quantified by RT-PCR,and the results indicated the existence of complexregulatory mechanisms between the miRNAs and their targets. Eight target cleavage sites wereverified by RNA ligase-mediated rapid 5’ end amplification. These results provide valuableinformation on the composition,expression and function of miRNAs in O. longistaminata,andwill aid in understanding the molecular mechanisms of rhizome development.[返回]
下载频数:8 上传时间:2014-06-19
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论文题目:Diversification of primary gene pool through introgression of resistance to foliar diseases from synthetic amphidiploids to cultivated groundnut (Arachis hypogaea L.)
全部作者: Varsha Kumaria,b,M.V.C. GowdaaJ Vinod Tasiwalc,Manish K. Pandeyb,Ramesh S. Bhatd,Nalini Mallikarjunab,Hari D. Upadhyayab,Rajeev K. Varshneyb,c,d
期刊名称:作物学报(英文版) 2014年第2,3期
论文摘要:Groundnut (Arachis hypogaea L.) is widely grown and consumed around the world and is considered to have originated from a single hybridization event between two wild diploids. The utilization of wild germplasm in breeding programs has been restricted by reproductive barriers between wild and...[查看全部]
论文摘要:Groundnut (Arachis hypogaea L.) is widely grown and consumed around the world and is considered to have originated from a single hybridization event between two wild diploids. The utilization of wild germplasm in breeding programs has been restricted by reproductive barriers between wild and cultivated species and technical difficulties in making large numbers of crosses. Efforts to overcome these hurdles have resulted in the development of synthetic amphidiploids,namely ISATGR 278-18 (Arachis duranesis x Arachis batizocoi) and ISATGR 5B (Arachis magna x A. batizocoi),which possess several desirable traits,including resistance to foliar diseases that generally cause huge yield losses annually in groundnut growing areas of Asia,America,and Africa. With an objective to improve foliar disease resistance,the primary gene pool was diversified by introgressing foliar disease resistance in five cultivated genotypes (ICGV 91114,ICGS 76,ICGV 91278,JL 24,and DH 86) from synthetic amphidiploids using a backcross breeding approach. Several introgression lines with resistance to two foliar diseases (rust and late leaf spot) were identified with levels of resistance equal to the donors. These backcross derived lines have shown a wide range of variation for several morphological and agronomic traits. These lines,after further evaluation and selection,can serve as donors in future breeding programs aimed at developing improved cultivars with desirable agronomic traits,high resilience to biotic/abiotic stresses and a broadened genetic base.[返回]
下载频数:9 上传时间:2014-06-19
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论文题目:Genetic characterization and linkage disequilibrium mapping of resistance to gray leaf spot in maize(Zea mays L.)
全部作者: Liyu Shia,b,c,Xiangling lva,b,c,Jianfeng Wenga,Hanyong Zhuc,Changlin Liua,Zhuanfang Haoa,Yu Zhoua,Degui Zhanga,Mingshun Lia,Xiaoke Cia,Xinhai Lia,*,Shihuang Zhanga,b*
期刊名称:作物学报(英文版) 2014年第2,3期
论文摘要:Gray leaf spot (GLS),caused by Cercospora zeae-maydis,is an important foliar disease of maize(Zea mays L.) worldwide,resistance to which is controlled by multiple quantitative trait loci (QTL).To gain insights into the genetic architecture underlying the resistance to this disease,anassociation...[查看全部]
论文摘要:Gray leaf spot (GLS),caused by Cercospora zeae-maydis,is an important foliar disease of maize(Zea mays L.) worldwide,resistance to which is controlled by multiple quantitative trait loci (QTL).To gain insights into the genetic architecture underlying the resistance to this disease,anassociation mapping population consisting of 161 inbred lines was evaluated for resistance toGLS in a plant pathology nursery at Shenyang in 2010 and 2011. Subsequently,a genome-wideassociation study,using 41,101 single-nucleotide polymorphisms (SNPs),identified 51 SNPssignificantly (P < 0.001) associated with GLS resistance,which could be converted into 31 QTL. Inaddition,three candidate genes related to plant defense were identified,including nucleotide-binding-site/leucine-rich repeat,receptor-like kinase genes similar to those involved in basaldefense. Two genic SNPs,PZE-103142893 and PZE-109119001,associated with GLS resistance inchromosome bins 3.07 and 9.07,can be used for marker-assisted selection (MAS) of GLSresistance. These results provide an important resource for developing molecular markersclosely linked with the target trait,enhancing breeding efficiency.[返回]
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论文题目:Effects of exogenous ABA application on post-anthesis dry matter redistribution and grain
全部作者: Dongqing Yanga,b,Yongli Luoa,b,Yingli Nia,b,Yanping Yina,Weibing Yanga,Dianliang Penga,Zhengyong Cuia,Zhenlin Wanga*
期刊名称:作物学报(英文版) 2014年第2,3期
论文摘要:The objective of this study was to investigate whether and how exogenous abscisic acid (ABA) isinvolved in mediating starch accumulation in the grain and redistribution of carbohydratesduring grain filling of two wheat cultivars with different staygreen characteristics. At bloomingstage,plants of...[查看全部]
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论文题目:Rank correlation among different statistical models in ranking of winter wheat genotypes
全部作者: Mozaffar Roostaeia,Reza Mohammadib,*,Ahmed Amric
期刊名称:作物学报(英文版) 2014年第2,3期
论文摘要:Several statistical methods have been developed for analyzing genotype x environment (GE)interactions in crop breeding programs to identify genotypes with high yield and stabilityperformances. Four statistical methods,including joint regression analysis (JRA),additivemean effects and...[查看全部]
论文摘要:Several statistical methods have been developed for analyzing genotype x environment (GE)interactions in crop breeding programs to identify genotypes with high yield and stabilityperformances. Four statistical methods,including joint regression analysis (JRA),additivemean effects and multiplicative interaction (AMMI) analysis,genotype plus GE interaction(GGE) biplot analysis,and yield-stability (YSi) statistic were used to evaluate GE interaction in20 winter wheat genotypes grown in 24 environments in Iran. The main objective was toevaluate the rank correlations among the four statistical methods in genotype rankingsfor yield,stability and yield-stability. Three kinds of genotypic ranks (yield ranks,stabilityranks,and yield-stability ranks) were determined with each method. The results indicated thepresence of GE interaction,suggesting the need for stability analysis. With respect to yield,the genotype rankings by the GGE biplot and AMMI analysis were significantly correlated(P < 0.01). For stability ranking,the rank correlations ranged from 0.53 (GGE-YSi;P < 0.05) to0.97 (JRA-YSi;P < 0.01). AMMI distance (AMMID) was highly correlated (P < 0.01) with varianceof regression deviation (S2di) in JRA (r = 0.83) and Shukla stability variance (a2) in YSi (r = 0.86),indicating that these stability indices can be used interchangeably. No correlation was foundbetween yield ranks and stability ranks (AMMID,S2di,a2,and GGE stability index),indicatingthat they measure static stability and accordingly could be used if selection is based primarilyon stability.[返回]
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论文题目:Development of highly glyphosate-tolerant tobacco by coexpression of glyphosate acetyltransferase gat and EPSPS G2-aroA genes
全部作者: Baoqing Duna,b,Xujing Wanga,Wei Lub,Ming Chenb,Wei Zhangb,Shuzhen Pingb,Zhixing Wangb,Baoming Zhanga,Min Linb
期刊名称:作物学报(英文版) 2014年第2,3期
论文摘要:The widely used herbicide glyphosate targets 5-enolpyruvylshikimate-3-phosphate synthase(EPSPS). Glyphosate acetyltransferase (GAT) effectively detoxifies glyphosate by N-acetylation.With the aim of identifying a new strategy for development of glyphosate-tolerant crops,theplant expression vector...[查看全部]
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论文题目:SSR genetic linkage map construction of pea (Pisumsativum L.) based on Chinese native varieties
全部作者: Xuelian Suna,b,Tao Yanga,b,Junjie Haoc,Xiaoyan Zhangc,Rebecca Fordd,Junye Jianga,b,Fang Wanga,b,Jianping Guana,b,Xuxiao Zonga,b*
期刊名称:作物学报(英文版) 2014年第2,3期
论文摘要:Simple sequence repeat (SSR) markers have previously been applied to linkage mapping of the pea (Pisum sativum L.) genome. However,the transferability of existing loci to the molecularly distinct Chinese winter pea gene pool was limited. A novel set of pea SSR markers was accordingly developed....[查看全部]
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