超(亚)临界流体色谱拆分萘普生对映体
首发时间:2004-03-25
摘要:本文以Kromasil CHI-TBB柱为手性固定相,异丙醇为改性剂,用超(亚)临界流体色谱法在温度293~323K和压力9.4~21.3 MPa的实验范围内拆分萘普生对映体。考察了温度、压力和改性剂浓度对分离因子、容量因子和分离度的影响。压力恒定时,分离因子的对数lnα与1/T都成简单的线性关系。计算了拆分过程中,对映体焓变差值和熵变差值。超(亚)临界流体色谱法拆分萘普生对映体属于焓控过程,较低的温度有利于对映体的分离。实验范围内的最佳分离条件为:压力9.4MPa,温度293K,流动相中异丙醇和CO2之比为11:89(w/w)。
关键词: 萘普生 手性拆分 超(亚)临界流体色谱法
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Resolution of naproxen enantiomers by supercritical (or subcritical) fluid chromatography
Abstract:A supercritical (or subcritical) fluid chromatography method for the resolution of naproxen enantiomers on the Kromasil CHI-TBB chiral stationary phase and CO2 with isopropanol as mobile phase has been studied. The effect of temperature, pressure and isopropanol content in CO2 on capacity factor, separation factor and resolution was investigated in the ranges of temperature 293~323K and pressure 12.0~21.0 MPa. The plots of lnα versus 1/T are straight lines. The differences of enthalpy changes and entropy changes for the transfer of enantiomers from the mobile phase to the stationary phase were calculated. The enthalpic contribution to the overall enantiomer transfer energy is generally more important than the entropic contribution in the temperature range examined, and lower temperature is better for chiral resolution of naproxen. The optimal operation conditions are 293K, 9.4MPa and the isopropanol content in CO2 11:89(mass/mass).
Keywords: Naproxen, Chiral Resolution, Supercritical (or Subcritical) Fluid Chromatography.
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No.5317778108018217****
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