链式聚合酶反应芯片研究进展
首发时间:2004-04-12
摘要:MEMS技术的发展使器件微小化,这必将对医疗和生物分析领域产生重要的影响,为了应付快速DNA分子扩增的需要,全世界许多研究组织已经陆续研发了一系列的微型反应仪器,本文通过对近期PCR生物芯片发展的回顾,对比了两种类型的PCR芯片,分析了影响PCR反应的主要因素,对于当前在PCR反应器件发展中占据主流技术和设计理念进行了阐述,并展望其未来的主要研究方向。
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Flow-through polymerase chain reactions in chip thermocyclers
Abstract:The miniaturization of analytical devices by micromachining technology is destined to have a major impact on medical and bioanalytical fields. To meet the current demands for rapid DNA amplification, various instruments and innovative technologies have been introduced by several groups in recent years. In this review the development of a variety of devices and components for performing DNA amplification as well as the comparison of batch-process thermocyclers with reaction chambers and flow-through devices for different purposes are discussed. We propose an overview of layouts for batch-process thermocyclers with different parallelization of reaction chambers and also of different designs of continuous flow thermocycling chips, paying particular attention to the parameters which influence the efficiency of such chip devices. Finally we point out some recent issues for applications in the field of clinical diagnostics.
Keywords: Polymerase Chain Reaction,Chamber,Continuous-flow Reaction Elements,MEMS
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No.5917421108173179****
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