Effect of Low Dose Ionizing Radiation on the Expression of DNA TopoisomeraseⅡα
首发时间:2004-06-11
Abstract:DNA topoisomeraseⅡα(TOPOⅡα) is a nuclear enzyme involved in chromosome segregation and other essential cellular processes. IHC and Western blot were used to determined the changes of TOPO II a expression and of its behavior in HeLa cells irradiated withγ-rays(≤2 Gy). A transient and significant decrease of TOPOⅡαafter low doseγ-rays exposure was detected: the decrease began at 4h after irradiation, reached to the lowest level at 12h, and began to return at 16h. Given low dose γ-rays, the level of decrease added as dose was added. The level of decrease after multiple irradiation was higher than that after single irradiation. This observation indicates that the decrease of TOPOⅡαafterγ-rays is time-dependent and dose-dependent, and the effect of γ-rays can add up. So the change of TOPOⅡαexpression may be related to chromosome non-disjunction.
keywords: ionizing radiation topoisomerase Ⅱα Down syndrome
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低剂量电离辐射对DNA拓朴异构酶Ⅱα表达的作用
摘要:目的 探讨低剂量电离辐射引起DNA拓扑异构酶Ⅱα(TOPOⅡα)的表达变化。方法 用免疫细胞化学染色和Western blot的方法,检测了Hela细胞在1Gyγ-射线照射后的时间效应和1-5Gy的剂量效应,以及小剂量的多次累积效应。结果 1Gyγ-射线照射后4小时TOPOⅡα开始下降,12小时降为最低,16小时开始回升;在1-5Gy时,随剂量增加,TOPO IIα的表达下降逐渐明显;且较小剂量具多次累积效应。结论 低剂量的电离辐射引起的TOPO IIα的表达变化具时间效应,剂量效应和多次累积效应。TOPO IIα的表达变化可能与染色体的不分离有关。
关键词: 电离辐射 拓朴异构酶Ⅱα
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