马红球菌4-2胆固醇氧化酶的急性毒理学研究
首发时间:2005-07-22
摘要:马红球菌4-2产生的胆固醇氧化酶应用于降解食品原料中的胆固醇,效果明显,降解率大于40%。为了进一步研究其安全性,选用霍恩氏法测定半数致死量LD50,结果证明胞内酶提取液的经口LD50大于21500mg/kg,属于无毒级别,有必要进入后续阶段的毒理学试验;活菌制剂在1.72×109个/ml剂量以下不会引起急性毒性反应。考虑到安全可靠性和简化操作,生产中应放弃使用活菌制剂,重点深入研究胞内酶的安全性,为生产实践提供参考。
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Acute Toxicology Research on the Cholesterol Oxidase Produced by Rhodococcus equi 4-2
Abstract:The cholesterol oxidase produced by Rhodococcus equi 4-2 was approved to be efficient to decompose the cholesterol in the raw materials of foods. To verify whether it was safe or not in food processing, an acute toxicology experiment was carried out. The results of Horn’s Method showed that LD50 of the endoenzyme extraction was higher than 21500mg/kg and thus was classified as no toxicity, while the microbe cells hadn’t begotten the acute toxic effects with the biomass less than 1.72×109cfu/ml. For the further safety consideration and simplifying the operation, the endoenzyme extraction should be investigated more and put into adoption.
Keywords: cholesterol oxidase acute toxicology
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No.2503165951121995****
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