New amperometric glucose biosensor by entrapping glucose oxidase into chitosan/nanoporous ZrO2/multiwalled carbon nanotubes nanocomposite film
首发时间:2005-11-30
Abstract:A new nanocomposite material for construction of glucose biosensor was proposed. The biosensor was formed by entrapping glucose oxidase in chitosan (CHIT)/nanoporousZrO2/multiwalled carbon nanotubes (MWNTs) nanocomposite. In this biosensing thin film, MWNTs can effectively catalyze hydrogen peroxide and nanoporous ZrO2 can enhance the stability of the immobilized enzyme. The resulting biosensor provides a very effective matrix for the immobilization of Gox and exhibites a wide linear response range of 8 mmol·L-1 to 3 mmol·L-1 with a correlation coefficient of 0.994 for the detection of glucose. And the response time and detection limit (S/N=3) of the biosensor are determined to be 6 s and 3.5 mmol·L-1, respectively. Another attractive characteristic is that thebiosensor is inexpensive, stable and reliable.
keywords: nanocomposite, glucose oxidase, nanoporous ZrO2, multiwalled carbon nanotubes, chitosan, biosensors
点击查看论文中文信息
壳聚糖/纳米ZrO2/多壁碳纳米管纳米复合膜固定葡萄糖氧化酶构建新电流型葡萄糖生物传感器
摘要: 利用壳聚糖具有生物相容性,多壁碳纳米管对过氧化氢的高效电催化性及纳米ZrO2能提高固定酶的稳定性等特点,制备了一种新的壳聚糖/纳米ZrO2/多壁碳纳米管纳米复合材料,并将葡萄糖氧化酶包埋到此纳米复合膜中,构建了新的电流型葡萄糖生物传感器。这种生物传感器为葡萄糖氧化酶提供了一种生物相容性好的固定基质,可充分保持酶的活性和防止酶从电极表面泄漏。该生物传感器对葡萄糖具有快速灵敏的响应,其响应时间、线性范围和检测限分别为:6 s,8 mmol·L-1 ~ 3 mmol·L-1, 3.5 mmol·L-1。该生物传感器制备方法简单、稳定性好,在构建基于过氧化氢检测的酶生物传感器有着广泛的应用前景。
论文图表:
引用
No.3934116401133313****
同行评议
共计0人参与
勘误表
壳聚糖/纳米ZrO2/多壁碳纳米管纳米复合膜固定葡萄糖氧化酶构建新电流型葡萄糖生物传感器
评论
全部评论0/1000