太湖溶藻细菌的分离及评价
首发时间:2005-11-07
摘要:从太湖梅梁湾流域的除藻中试装置的人工介质上分离到一株溶藻细菌,确定种属后对其溶解铜绿微囊藻细胞和降解微囊藻毒素的效果进行研究,并应用新建立的实时荧光定量PCR法(RTQ-PCR)对人工介质上不同时空的细菌丰度进行检测。结果显示,经生理生化和分子生物学分析鉴定该株细菌为芽孢杆菌属;该株细菌具有较强的溶解铜绿微囊藻细胞和降解微囊藻毒素LR(MC-LR)功能,浓度为106个/mL的该芽孢杆菌在初始浓度为4.5×107个/mL铜绿微囊藻液中第16、32、48小时对藻细胞去除率分别为35%、67%和100%,在MC-LR初始浓度为2.649μg/L的藻液中第18、36、54和72小时藻毒素降解率分别为15%、29%、73%和100%。人工介质上该芽孢杆菌的细菌丰度具有一定的时空变化规律,7、8、9三个月芽孢杆菌属的丰度呈递增趋势。
关键词: 溶藻细菌 芽孢杆菌属 铜绿微囊藻 微囊藻毒素LR 实时荧光定量PCR
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Isolation and evaluation of algicidal bacteria from Taihu Lake
Abstract:An algicidal bacterium was isolated and identified from synthetic medium of a microcystin treatment apparatus in Meiliang Bay of Taihu Lake in this study. Its efficiency of algicidal activity to Microcystis and degradation to microcystin-LR were studied. The real-time quantitative PCR (RTQ-PCR) amplification method was established to quantitatively detect relative abundances of this bacterium in the environment. The results show that the bacterium can be identified as Bacillus sp by the analysis of morphological, physiological, biochemical property and 16S rRNA selective PCR (polymerase chain reaction). The significant effects of algicidal activity and degradation of microcystin-LR of the bacteria were found in the study. The 16, 32 and 48 hours aglae-lysing rates of Bacillus sp in BG11 algae medium were 35%,67% and 100%, respectively. With the initial concentration of 2.649μg/L, the microcystin-LR degradation rates of the bacterium after 18, 36, 54 and 72 hours exposure were 15%,29%,73% and 100%, respectively. The relative abundances of Bacillus sp in the synthetic medium of microcystin treatment apparatus in Meiliang Bay of Taihu Lake had the tendency of increase among July, August and September.
Keywords: algicidal bacteria Bacillus sp Microcystis aeruginosa microcystin-LR real-time quantitative PCR
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