TTC法检测牛奶中的抗菌药物
首发时间:2006-03-10
摘要:针对用TTC法检测牛奶抗菌药物残留过程中因为在被检乳样中加入指示菌的数量不稳定而影响检测的问题,本研究增设了菌液稀释度试验以确定加入的菌液量。同时,采用TTC法对奶牛场常用的几种抗菌药物检测其乳中最低检测浓度。结果是青霉素3μg/kg,链霉素660μg/kg,庆大霉素620μg/kg,卡那霉素6200μg/kg,红霉素30μg/kg,环丙沙星500μg/kg,土霉素100μg/kg,金霉素100μg/kg,磺胺嘧啶100μg/kg。本研究用TTC法对青霉素、红霉素、土霉素、金霉素和磺胺嘧啶的检测灵敏度均达到农业部规定的牛乳中兽药残留最高限量要求。TTC法具有简便、价廉的优点,并且所检测的抗菌素范围较广,符合我国奶牛场中使用抗菌药物种类较多的现状
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Determination of Antibacterial Drug’s Residues in Bovine Milk with a TTC Test
Abstract:In determination of antibacterial drug’s residues in milk, the result is often influenced by the number of indicator bacteria added into a milk sample when a TTC test is used. Therefore, a test for determination of the number of indicator bacteria was proposed before a TTC test was carried out. This study was designed to determine the minimum concentration of 9 common antibacterial drugs in bovine milk detectable by the TTC test. The results indicated that the limits for detection of penicillin, streptomycin, gentamicin, kanamycin, erythromycin, ciprofloxacin, oxytetracycline, aureomycin and sulfadiazine were 3μg/kg, 660μg/kg, 620μg/kg, 6200μg/kg,30μg/kg, 500μg/kg,100μg/kg,100μg/kg and 100μg/kg, respectively. The detectable limits for penicillin, erythromycin, oxytetracycline, aureomycin and sulfadiazine were equal to or bellow the Minimum Residue Limit Standards set by the Ministry of Agriculture of China. As the TTC test was simple and cheap with a broad spectrum of drugs detectable, it is suitable for the current status of dairy farms where various antibacterial drugs are used for disease control.
Keywords: Milk TTC Antibacterial drugs Antibiotic Residue
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