妊娠高血压造成胎鼠及其出生后幼鼠海马细胞增殖下降和神经元减少的可能机制以及灵芝孢子的干预作用
首发时间:2006-04-06
摘要:目的 探讨妊娠高血压造成胎鼠及其生后幼鼠大脑海马细胞增殖下降和神经元减少的可能机制以及灵芝孢子的干预作用。方法 将40只SD妊娠大鼠分为正常对照组,L-NAME+蒸馏水组,L-NAME+精氨酸组和L-NAME+灵芝孢子组,采用免疫组化、Western-Blot、RT-PCR、流式细胞计数和电镜等技术对大脑海马组织进行检测。结果 应用L-NAME后,E21大脑海马低氧诱导因子-1a(HIF-1a)和血管表皮生长因子(VEGF)的表达增加,直到P30时仍有表达。E21海马微血管密度增高, P30海马毛细血管结构异常。E21海马细胞的增殖下降, P30海马神经元减少。应用灵芝孢子后, E21海马HIF-1a 和VEGF低水平表达,在P30时则未见表达。E21海马微血管密度达到正常水平, P30海马毛细血管结构正常。E21海马细胞增殖上升, P30海马神经元恢复性增多。结论 灵芝孢子能够干预妊娠高血压引起的胚鼠及其生后幼鼠大脑海马细胞增殖能力下降和神经元的减少。
关键词: 妊娠高血压; 微血管密度;细胞增殖;大鼠
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POSSIBLE MECHANISMS OF DECREASED CELL PROLIFERATION AND NEURONAL NUMBER AT THE HIPPOCAMPUS OF FETAL AND POSTNATAL RATS INDUCED BY GESTATIONAL HYPERTENSION
Abstract:Objective To investigate the possible mechanisms of decreased cell proliferation and neuronal number at the hippocampus of fetal and postnatal rats induced by gestational hypertension and the treatment effect of Ganoderma spore. Methods 40 SD pregnant rats were divided into four groups including normal control group, L-NAME +distilled water(DW) group, L-NAME+L-Arginine group and L-NAME+Ganoderma spore(GS) group. The hippocampal tissue of brain was detected by immunohistochemistry, Western-Blot, RT-PCR, flow cytometry and electron microscope. Results After the application of L-NAME, hypoxia inducing factor-1a(HIF-1a) and vascular endothelial growth factor(VEGF) expression were increased at the hippocampus of E21 brain and continued up to P30 brain. The microvessel density of E21 hippocampus was increased and the structural abnormalities of blood capillary at P30 hippocampus were showed. The cell proliferation was decreased at E21 hippocampus and so did the neuronal number at P30 hippocampus. With administration of Ganoderma spore, HIF-1a and VEGF were down-regulated at E21 hippocampus and were not detected at P30 hippocampus. The microvessel density of E21 hippocampus and blood capillary ultrastructure of P30 hippocampus recovered to normal status. The cell proliferation of E21 hippocampus and neuronal number of P30 hippocampus were recuperatively increased. Conclusion Ganoderma spore may treat the reduction of cell proliferation and neuronal number at the hippocampus of fetal and postnatal rats induced by gestational hypertension.
Keywords: Gestational hypertension Microvessel density Cell proliferation Rat
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妊娠高血压造成胎鼠及其出生后幼鼠海马细胞增殖下降和神经元减少的可能机制以及灵芝孢子的干预作用
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