原子力显微镜技术在超微生物力学测量中的应用研究
首发时间:2006-05-19
摘要:微观生物力学与生物学中几乎所有的物理化学过程都有着息息相关的联系,这些微观力的检测与表征能为生物学过程的阐明提供有力的证据。多年来人们一直尝试使用各种仪器对这些微观力进行测量。其中,最为成功的原子力显微镜是一个相当新颖并且十分有效的研究工具,在纳米范围内,它对生物过程的研究起着非常重要的作用。用它不仅可以在分子水平上对作用力进行测量,得到表面形状的图像,而且也可以用于高分子的解旋和空间构象力的研究,从而了解样品的空间构成,此外也可研究样品表面的其他物理特性如压弹性、粘弹性特性的研究。文章首先阐明了微观力学表征的生物学意义;接着强调了原子力显微镜探针与样品间相互作用的类型及其在生物力学量测量中的原理和过程;之后分别介绍了AFM在细胞间黏附力、生物分子间相互作用、单分子力谱等方面的应用成果;最后结合AFM的应用现状对微观生物力的AFM深入研究进行了展望。
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The Application of Atomic Force Microscopy to the Measurement of Biomechanics in Micro-scale
Abstract:There are the tight relations between biomechanics and nearly all of the physical and chemical process in the biological life, and these mechanical parameters can provide enough proofs for the life science. In the past years, scientists have been trying to measure these parameters with different equipments and methods, in which the atomic force microscopy may be the best and most successful equipment and techniques. As a novel tool in nanostructure of materials special in life science, atomic force microscope runs a key role in the life process, and it can get the surface topographies of the sample and much more about the physical and mechanic properties. Firstly, demonstrate the meanings of the token for biomechanics and then stress on the type of the interaction between stylus and sample surface; secondly, introduce the application of AFM in the cell, biomolecules and single molecule. And end with the prospecting and discussions for the deep researches to biomechanics in micro-scale with atomic force microscope.
Keywords: Atomic force microscope Biomechanics Single molecular force spectroscope
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No.6693560606114800****
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