Study of antioxidants extracted with supercritical carbon dioxide from Spirulina platensis

• 1、Nanjing Agricultural University

Abstract：The process was optimized for supercritical CO2 extraction of antioxidants from Spirulina platensis using response surface methodology. About 10.26 g/kg of extracts from Spirulina platensis could be obtained under the optimum conditions, viz., temperature 48℃, pressure 20 MPa, time 4 h. The antioxidant activity of the extracts, determined by inhibiting peroxidation of linoleic acid method, was a little lower compared with that of BHT and Trolox, but was much higher than α-tocopherol at 200th min. However, the activity of the extracts became similar to that of α-tocopherol, significantly lower than BHT and Trolox at 400th min. The components of the extracts were further analyzed, and the results showed that the extracts contained 85.1 g/kg of flavonoids, 77.8 g/kg of β-carotene, 113.2 g/kg of vitamin A, 3.4 g/kg of α-tocopherol, which may contribute greatly to its high antioxidant activity. The main components of the free fatty acid in the extracts were palmitic acid (35.32%), linolenic acid (21.66%), linoleic acid (20.58%).

Keywords： Spirulina platensis, supercritical carbon dioxide, extraction, antioxidant