一株产槲皮素类色素的银杏内生真菌的培养条件的研究
首发时间:2006-07-06
摘要:从银杏根部韧皮部分纯化出一株内生真菌Gh01可产橙黄色色素,经过鉴定主要成分为槲皮素类黄酮。本文初步鉴定了菌株,同时对发酵条件作了详细探讨:试验表明,该菌株属于青霉属,色素发酵培养基最佳配方为:马铃薯煮出液为基本培养基,外加2%葡萄糖,0.5%蛋白胨,0.1%氯化锌,培养温度为28℃,初始pH为6,培养时间120h。
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Study on a ginkgo biloba L.
Abstract:Gh01 strain was isolated from the phloem of the root of Ginkgo biloba L.and it could produce orange pigment. The pigment was identifided as quercetin glycoside. In this paper,we have studied all kinds of factors that influence on the product of orange pigment. Optimum culture conditions of Gh01 were potato decoction as the basic culture medium, glucose concentration 5%, peptone concentration 0.2%, cholridize zinc concentration 0.1%, 28℃, beginning pH 6. Under those conditions maximum pigment produced after 120h.
Keywords: Ginkgo biloba L, endophytic fungi, pigment, culture conditions
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No.7482585071152159****
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一株产槲皮素类色素的银杏内生真菌的培养条件的研究
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