一株喜温硫杆菌的分离及对金属硫化矿浸出的促进作用
首发时间:2006-07-07
摘要:从某煤矿废水分离纯化到一株中度嗜热嗜酸菌硫氧化细菌S2,根据其生理生化特性以及16Sr RNA序列测定初步确定为喜温硫杆菌。S2纯培养物分别和Leptospirillum ferrooxidan(Lf)纯菌以及Acidihiobacillus ferrooxidans(Af)纯菌混合浸出黄铜矿和黄铁矿的结果表明:S2的存在能明显促进Lf和Af对硫化矿的金属浸出率
关键词: 喜温硫杆菌 16Sr RNA序列 生物浸出 分离 硫化矿
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Isolation of a strain of Acidithiobacillus caldus and its promoting role in bioleaching of sulfide minerals
Abstract:A moderately thermophilic and acidophilic sulfur-oxidizing bacterium S2, was isolated from acid mine drainage. The bacterium is a motile, Gram-negative, rod-shaped, and measures 0.4 to 0.6 by 1 to 2 μm. It grows optimally at 42℃-45℃ and initial pH 2.5. The strain grows autotrophically by using elemental sulfur, sodium thiosulfate and potassium tetrathionate as energy sources. Growth does not occur in medium containing any of following energy sources: peptone, yeast extract powder, glucose, ferrous sulfate, pyrite and chalcopyrite. The morphological, biochemical and physiological characterization and analysis based on 16S rRNA gene sequence indicated that S2 is most closely related to Acidithiobacillus caldus. When S2 is in combinated with Leptospirillum ferrooxidans or Acidithiobacillus ferrooxidans as inocula in pyrite or chalcopyrite bioleaching system, the result showed that S2 could positively increase the metal leaching rate of metal sulfides ores. Scanning electron microscope analysis revealed that the surface of chalcopyrite was seriously destroyed in mixed culture systems.
Keywords: Acidithiobacillus caldus Bioleaching Isolation Sulfide mineral 16S rRNA
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