用牛血清蛋白芯片在表面等离子共振仪中进行谷胱甘肽与谷胱甘肽转移酶相互作用研究
首发时间:2006-09-28
摘要:简要说明 首先用牛血清蛋白修饰芯片,然后把谷胱甘肽固定于反应通道的牛血清蛋白芯片上,并将参考通道的牛血清蛋白表面作为参考表面,通过将谷胱甘肽转移酶注入到表面等离子共振仪的两通道中,对其相互作用进行实时研究,结果表明牛血清蛋白修饰过的芯片在这种使用方法中不仅谷胱甘肽能与其转移酶有效结合而且还能抑制蛋白质非特异相互作用,所测得的动力学常数为106与文献值相近。相同的试验用商业产品CM5(羧甲基葡聚糖表面)没得到好的结果。
关键词: 谷胱甘肽,谷胱甘肽转移酶,表面等离子共振仪,牛血清蛋白
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Investigation on the Interaction of Glutathione and Glutathione-S-Transferase with the BSA-bounded biochip Using Surface Plasmon Resonance
Abstract:A chip was modified with bovine serum albumin (BSA), then interaction between glutathione (GSH) immobilized on the top of BSA and glutathione-S-transferase (GST) was examined using surface plasmon resonance (SPR). The SPR results showed that BSA-modified chip was effective not only in binding the target proteins but also in suppressing the nonspecific binding (NSB) of proteins.
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No.8544777921159415****
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用牛血清蛋白芯片在表面等离子共振仪中进行谷胱甘肽与谷胱甘肽转移酶相互作用研究
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