芸薹属两个亚种间杂种光合作用优势及其机理

董德坤; 师恺; 曹家树

0
0
浏览
下载

摘要
关键词
基金信息
论文图表
同行评议
相关论文
评论

芸薹属两个亚种间杂种光合作用优势及其机理

首发时间：2006-11-24

董德坤 ¹ 师恺 ¹ 曹家树 ²

1、浙江大学蔬菜研究所，杭州310029
2、浙江大学蔬菜研究所，杭州

摘要：为了探讨芸薹属作物杂种是否具有光合作用优势及其机理，我们利用气体交换和叶绿素荧光技术系统地研究了‘矮脚黄’白菜和‘白蔓菁’芜菁及其正反交杂种的叶片光合作用的特性，并对卡尔文循环中的关键酶核酮糖1，5-二磷酸羧化/加氧酶（RuBisCO）进行了相对定量研究。在整个实验期间，杂种F1的净光合速率（Pn）显著高于双亲，具有较强的杂种优势。杂种气孔导度（Gs）也高于亲本，但其胞间CO2浓度（Ci）和气孔限制值（l）与亲本间没有明显的差异。杂种总叶绿素含量介于双亲之间，仅表现出微弱的中亲优势。杂种PSII光合电子传递量子效率（ΦPSII）及光合电子传递速率（ETR）显著高于亲本，其变化趋势和PSII反应中心开放程度（qP）一致。RuBisCO最大羧化速率（Vcmax）、最大电子传递速率（Jmax）和RuBisCO蛋白含量在杂种中均有所增加。综上所述气孔导度、叶绿素含量和光呼吸均不是杂种光合优势产生的主要原因。光合优势的产生很可能是由于碳固定效率的增加反馈上调了光合电子传递速率而引起的。

关键词：芸薹属；亚种间杂种；光合作用；叶绿素荧光；杂种优势；核酮糖1 5-二磷酸羧化/加氧酶

For information in English, please click here

The Photosynthetic Heterosis and Its Mechanisms of an Inter-Subspecific Hybrid between Brassica campestris ssp. chinensis and B. campestris ssp. rapifera

Dong Dekun ¹ Shi Kai ¹ Cao Jiashu ²

1、Institute of Vegetable Science, Zhejiang University, Hangzhou 310029
2、310029Institute of Vegetable Science, Zhejiang University, Hangzhou 310029

Abstract：To investigate whether hybrid of Brassica crops has heterosis in photosynthetic capacity and reveal the mechanisms involved, the reciprocal inter-subspecific hybrids between Brassica campestris ssp. chinensis ‘Aijiaohuang’ and B. campestris ssp. rapifera ‘Baimanjing’, and their parents were used to analyze photosynthetic characteristics by simultaneous measurement of leaf gas exchange and chlorophyll fluorescence parameters. The ribulose-1, 5-bisphosphate carboxylase/oxygenase (RuBisCO), a key enzyme in the Calvin cycle was also investigated accordingly. Results showed that the net CO2 assimilation rates (Pn) of hybrids were significantly higher than that of their parents in the experiment, leading to a strong heterosis in photosynthesis. Hybrids also increased stomatal conductance (Gs) but had no influence on intercellular CO2 concentration (Ci) and stomatal limitation (l). The concentrations of total chlorophyll in the hybrids were between their parents with only a weak mid-parent heterosis. Hybrids significantly increased the quantum efficiency of PSII photochemistry (ΦPSII) and the electron transport rate (ETR), which were accompanied by a similar increase in photochemical quenching (qP). Additionally, hybrids also had evident heterosis in the maximum carboxylation rate of RuBisCO (Vcmax), maximum electron transport rate (Jmax), and the RuBisCO protein content. It was concluded that the increase of photosynthesis in the hybrids were not accounted by the changes in stomatal conductance, chlorophyll content or photorespiration. The photosynthetic heterosis might be mainly attributed to the improved efficiency of photosynthetic carbon fixation in the Calvin cycle which resulted in an up-regulation of electron transport.

Keywords： Brassica Inter-subspecific hybrid Photosynthesis Chlorophyll fluorescence Heterosis RuBisCO

基金：

1. 教育部博士点基金（20030335117）

论文图表：

引用

导出参考文献

.txt

.ris

.doc

董德坤，师恺，曹家树. 芸薹属两个亚种间杂种光合作用优势及其机理[EB/OL]. 北京：中国科技论文在线 [2006-11-24]. https://www.paper.edu.cn/releasepaper/content/200611-681.

No.9862879181164342****

同行评议

共计0人参与

全部评论

0/1000

论文编号	200611-681
论文题目	芸薹属两个亚种间杂种光合作用优势及其机理
文献类型
收录期刊	上传封面中文期刊英文期刊期刊名称（中文）期刊名称（英文）年，卷（）上传封面中文专著英文专著书名（中文）书名（英文）出版地出版社出版年上传封面中文译著英文译著书名（中文）书名（英文）出版地出版社出版年上传封面中文论文集英文论文集编者.论文集名称（中文） [c]. 出版地出版社出版年， - 编者.论文集名称（英文） [c]. 出版地出版社出版年，- 上传封面中文文献英文文献期刊名称（中文）期刊名称（英文）日期-- 在线地址http:// 上传封面中文文献英文文献文题（中文）文题（英文）出版地出版社,出版日期-- 上传封面中文文献英文文献文题（中文）文题（英文）出版地出版社,出版日期--
英文作者写法：中外文作者均姓前名后，姓大写，名的第一个字母大写，姓全称写出，名可只写第一个字母，其后不加实心圆点“.”, 作者之间用逗号“，”分隔，最后为实心圆点“.”, 示例1：原姓名写法：Albert Einstein,编入参考文献时写法：Einstein A. 示例2：原姓名写法：李时珍；编入参考文献时写法：LI S Z. 示例3：YELLAND R L,JONES S C,EASTON K S,et al.