牛血清蛋白稀溶液的粘度研究
首发时间:2007-01-10
摘要:本文测量了15-40℃之间不加盐的牛血清蛋白水溶液的粘度随浓度的变化,发现:牛血清蛋白是一种“硬蛋白”,从特性粘数估算出的分子尺寸随温度的变化很小,它在毛细管玻璃内表面的吸附量很低。自缔合常数在所有温度下都为零,证实了它难于聚集的特点。牛血清分子吸附后会发生构象变化,表现为分子变“扁”。当温度降低吸附量增大后,吸附层的厚度出现了先减小后增大的现象,考虑到牛血清蛋白在界面以单层吸附为主的特点,我们推测由于分子在界面上随着温度降低进一步铺展,导致吸附层的平均高度降低。其后吸附层的厚度的增大是由于单层吸附量达到最大饱和值后开始出现多层吸附所致。有效吸附层厚度远大于分子在溶液中的尺寸,是由于分子之间存在库仑斥力,导致吸附分子和流动分子之间存在一个排斥层。
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Determiantion of the Viscosity for BSA in Dilute aqueous Solution
Abstract:Viscosities of BSA in dilute aqueous solution at different temperatures were carefully measured. The upward phenomena of the apparent viscosity of BSA solutions was attributed to the adsorption of the solute onto the inner wall of the glass capillary and a corrected viscosity equation was used to deal with the obtained viscosity data. Five viscosity parameters have been obtained to describe the state of casein molecules in the solution and on the interface. It seemed that the size of BSA molecules in solution changed little with temperature and the adsorption layer thickness varied irregularly with the adsorption amount.
Keywords: viscosity BSA adsorption
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