牙鲆消化道发育的组织学观察
首发时间:2007-03-26
摘要:为研究牙鲆仔鱼消化道发育的特点,定期取材,通过组织学的方法,在解剖镜和光学显微镜下观察牙鲆出膜前后、出膜15天、30天、50天消化道发育情况。出膜后3天内,消化道不断延长并且和卵黄共存,肠开始分化。卵黄也越来越小,第5天卵黄基本消失,肝脏初步发育,位于肠的上方。出膜后15天,变态发育开始,消化道已经分化为口、咽、食道、胃前体、肠和后肠。胰脏位于肠的环绕中。杯状细胞在消化道大多数部位都有分布。5-羟色氨细胞、胃泌素细胞和P物质细胞三种内分泌细胞很少且形状不规则。第30天,消化道分化完全,胃上皮发育成柱状上皮,杯状细胞减少,可见胃腺,标志胃成熟。胃盲囊也出现。内分泌细胞在胃肠中增多,但个体间存在一定的差异。第50天,幼鱼消化道发育比较成熟,管壁的结构完整,三种内分泌细胞分布部位出现分化,幼鱼的肝脏和胰脏仍在进一步发育。
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Development of the digestive tract in larval Japanese flounder
Abstract:Histological changes of the digestive tract were studied in Japanese flounder(Paralichthys olivaceus) from hatching until day 50. Samples were taken after hatching, then of 5, 8, 15, 30 and 50 days post-hatch. During the first 3 days after hatching, the digestive tract was lengthened and coexist with the sac yolk. The mocosae began to proliferate and differentiate. The yolk became smaller and smaller after the time of mouth opening. The liver is situated on top of the intestine. From 15 days, they were metamorphosing. At the beginning of the metamorphosis, the digestive tract could be divided into buccal cavity, pharynx, oesophagus, post-oesophgeal swelling, intestine, and rectum. The exocine pancreas is located within the intestinal loop. Goblet cells were present in most places of the tract. Very few endocrine cells could be detected. By day 30, the posterior zone of the oesophagus had a marked increase in goblet cells density and mucosal folding. Gastric gland, columnar epithelium of the post-oesophgeal swelling allowed it to be defined as a stomach. Goblet cells were also disappeared in the post-oesophgeal swelling. Pyloric caeca extended from the anterior intestine. Many endocrine cells could be found in stomach and intestine. These cells were brown staining. Some cells were round like a finger ring, others were long, opening via a duct to the digestive lumen. By day 50, the structure of the digestive tract was mature like that of adult fishes. Serotonin cells were distributed in the stomach, gastrin and P substance cells were distributed in the pyloric caeca and foregut.
Keywords: Paralichthys olivaceus;digestive tract development immunohistochemistry
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No.1167710162811749****
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