变形链球菌gtf在不同蔗糖浓度下的差异性表达
首发时间:2007-07-24
摘要:目的 检测具有不同产胞外多糖能力的变形链球菌株在不同培养条件下产胞外多糖(EPS)的能力及其与gtfA,B,C,D表达变化的关系。材料与方法 选取高产糖与低产糖的临床分离株各10株及标准株UA159,用蒽酮法分别检测其在1%蔗糖和2%蔗糖培养条件下产EPS的能力。再以real-time PCR方法检测在这些条件下与变链产糖能力密切相关的毒力因子gtfA,B,C,D的表达变化。结果 蔗糖在各菌株均能诱导EPS的产生。在1%蔗糖浓度下,高产糖株与低产糖株的gtfA,B,C,D表达均有不同程度升高。在2%蔗糖浓度下,低产糖株的gtfB,C,D表达均不同程度降低,gtfA略有升高;高产糖株的gtfB,D表达略有升高,gtfA,C略有降低。结论gtfs的表达水平与变形链球菌的致龋力密切相关。
关键词: 变形链球菌 葡萄糖基转移酶 real-time PCR
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Differential and Qutantitive Analyses of mRNA Expression of gtfs from S mutans
Abstract:Objective To determine the expression level of each gtf under various conditions and to find the relationship between gtf expression levels with the ability to produce extracellular polysacchradides (EPS) in different strains of S mutans. Material and Methods 20 strains S mutans of clinical isolations with different EPS producibility and UA159 were selected, their ability to produce EPS under 1% sucrose and 2% sucrose were tested. Then in two strains, the relative quantity of gtfA,B,C,D’s mRNA were examined by real-time PCR methods. Results In all strains, the quantity of EPS were prominently enhanced by sucrose. The expression levels of gtfA,B,C,D were increased in 1%sucrose in both strains. In 2% sucrose, expression levels of gtfB,C,D were decreased and gtfA was increased in the strain which produced less EPS. gtfB,D were decreased and gtfA,C were increased in the strain which produced more EPS. Conclusion S mutans produces GTFs at an optimal ratio to adhere to the tooth surface.
Keywords: S mutans gtf real-time PCR
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