甘油预处理对冻干红细胞恢复率及抗氧化酶活性的影响

周新丽; 何晖; 刘宝林; 华泽钊; 陈颖

0
0
浏览
下载

摘要
关键词
基金信息
论文图表
同行评议
相关论文
评论

EFFECTS OF GLYCEROL PRETREATMENT ON RECOVERY AND ANTIOXIDANT ENZYME ACTIVITIES OF LYOPHILIZED RED BLOOD CELLS

首发时间：2007-09-29

Zhou Xinli ¹ He Hui ¹ Liu Baolin ¹ Hua ZeChao ¹ Chen Ying ²

1、Institute of Biothermal Sciences, University of Shanghai for Science and Technology
2、Institute of Blood test，Shanghai Antu Hospital

Abstract：Successful storage of red blood cells (RBCs) by freeze-drying technique has important implications in blood transfusion and clinical medicine. We presented a method of preservation RBCs by pretreating them with glycerol solution and then freeze-drying. The effects of glycerol pretreatment on recovery and antioxidant enzyme activities of lyophilized RBCs were investigated. RBCs pretreated in 40% glycerol resulted in 55.3±4.26% numerical recovery and 53.5±3.85% hemoglobin recovery, which were significantly different from control group (P<0.01). Superoxide dismutase(SOD), catalase (CAT) and glutathione peroxidase (GSH-PX) activities of 40% glycerol pretreated, freeze-dried RBCs almost remained fully active after freeze-drying. After 180 days storage, they decreased by 25.6%，21.5% and 18.4%, respectively, which are significantly lower than those of no glycerol pretreated, freeze-dried RBCs (P<0.1). These data demonstrated that glycerol pretreatment had beneficial effects on the recovery and antioxidant enzyme activities of lyophilized RBCs.

keywords： Glycerol； pretreatment Freeze-drying Red Blood Cells

点击查看论文中文信息

甘油预处理对冻干红细胞恢复率及抗氧化酶活性的影响

周新丽 ¹ 何晖 ¹ 刘宝林 ¹ 华泽钊 ¹ 陈颖 ²

1、上海理工大学生物系统热科学研究所
2、上海安图医院血液检验科

摘要：用冷冻干燥技术保存红细胞在输血和临床医学上有重要的应用价值。我么提出一种用甘油溶液预处理红细胞然后冷冻干燥的保存方法，研究了甘油预处理对冻干红细胞恢复率及抗氧化酶活性的影响。经40%甘油预处理冻干的红细胞数值恢复率为55.3±4.26%，血红蛋白恢复率为53.5±3.85%，显著高于未经甘油预处理的控制组(P<0.01)。经40%甘油预处理冻干的红细胞超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-PX）几乎保持了完全的活性。储存180天后，它们的活性分别降低25.6%，21.5%和18.4%,显著低于未经甘油预处理冻干的红细胞组（P<0.1）。这些数据表明甘油预处理有助于保存冻干红细胞的恢复率及抗氧化酶活性。

关键词：甘油预处理冻干红细胞

基金：

1. Specialized research fund for the doctoral program of higher education，Science Foundation for training excellent young teacher of Shanghai，Science Fou（20050252002，21035，06SG47）

论文图表：

引用

导出参考文献

.txt

.ris

.doc

Zhou Xinli ,He Hui ,Liu Baolin ,et al. EFFECTS OF GLYCEROL PRETREATMENT ON RECOVERY AND ANTIOXIDANT ENZYME ACTIVITIES OF LYOPHILIZED RED BLOOD CELLS[EB/OL]. Beijing:Sciencepaper Online[2007-09-29]. https://www.paper.edu.cn/releasepaper/content/200709-628.

No.1543514809611910****

同行评议

共计0人参与

全部评论

0/1000

论文编号	200709-628
论文题目	甘油预处理对冻干红细胞恢复率及抗氧化酶活性的影响
文献类型
收录期刊	上传封面中文期刊英文期刊期刊名称（中文）期刊名称（英文）年，卷（）上传封面中文专著英文专著书名（中文）书名（英文）出版地出版社出版年上传封面中文译著英文译著书名（中文）书名（英文）出版地出版社出版年上传封面中文论文集英文论文集编者.论文集名称（中文） [c]. 出版地出版社出版年， - 编者.论文集名称（英文） [c]. 出版地出版社出版年，- 上传封面中文文献英文文献期刊名称（中文）期刊名称（英文）日期-- 在线地址http:// 上传封面中文文献英文文献文题（中文）文题（英文）出版地出版社,出版日期-- 上传封面中文文献英文文献文题（中文）文题（英文）出版地出版社,出版日期--
英文作者写法：中外文作者均姓前名后，姓大写，名的第一个字母大写，姓全称写出，名可只写第一个字母，其后不加实心圆点“.”, 作者之间用逗号“，”分隔，最后为实心圆点“.”, 示例1：原姓名写法：Albert Einstein,编入参考文献时写法：Einstein A. 示例2：原姓名写法：李时珍；编入参考文献时写法：LI S Z. 示例3：YELLAND R L,JONES S C,EASTON K S,et al.