Effects of Acupuncture on Hepatic IRS-1mRNA Expression in Type 2 Diabetes Mellitus Model Rats

• 1、Second Clinical Medical College of Nanjing TCM

Abstract：Objective: To research the effects of acupuncture on the expression of hepatic IRS-1mRNA in type 2 diabetes mellitus(T2DM) model rats and the comparison of regulating action on expression of hepatic IRS-1mRNA in T2DM rats between acupuncture and Glyburide. Method: 50 of 100 SD male rats fed with high fat feedstuff became fat rats, which were injected in abdomen with Streptozotocin(25mg/kg) to make T2DM rat models. Then T2DM rats(18) were Stochastically devided into Glyburide group(6), acupuncture group(6) and model group(6). After treatment for 4 weeks, fast blood sugar(FBS) was examined by active blood sugar meter and fast insulin(FINS) was examined by radioimmunoassav(RIA) and expression of hepatic IRS-1mRNA was examined by real-time RT PCR, the results were compared with normal rats. Result: After treatment, the level of FBS in acupuncture and Glyburide group are obviously lower than model group(P<0.05); the level of FINS in acupuncture is markedly lower than model group(P<0.05), the level of FINS in Glyburide group is also lower than model group but there has no statistical meaning between them(P>0.05). The IRS-1mRNA of model group is a quater of normal group; the IRS-1mRNA of Glyburide group is 1.23 times as that of the normal group ; the IRS-1mRNA of acupuncture group is 2.83 times as that of the normal group. Conclusion: Acupuncture and Glyburide also can improve the expression of hepatic IRS-1mRNA in T2DM rats, but acupuncture’s effect is better than Glyburide.

Keywords： type 2 diabetes mellitus IRS-1mRNA real-time RT PCR acupuncture.