普通小麦-簇毛麦2V染色体端体异附加系的选育与鉴定
首发时间:2007-12-13
摘要:簇毛麦(Haynaldia villosa)具有抗多种病害、耐旱、耐寒等优良性状,是可供小麦改良利用的优良遗传资源。本研究综合利用根尖细胞有丝分裂中期染色体Giemsa C-分带、花粉母细胞减数分裂中期I染色体构型分析、荧光原位杂交(Genomic in situ hybridization, GISH)和分子标记等技术,从普通小麦-簇毛麦2V(2D)异代换系与含有Ph抑制基因的中国春高配对材料(Pairing homoeologous inhibitor, PhI)的杂交后代中选出分别含有簇毛麦2V长臂和短臂的普通小麦-簇毛麦2V端体异附加系。将控制护颖颖脊刚毛性状的基因进一步定位在簇毛麦2V染色体的短臂上。同时筛选出可以追踪2V染色体短臂的SSR标记wmc25。
关键词: 普通小麦; 簇毛麦; 端体异附加系; 染色体C-分带 荧光原位杂交 SSR
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Development and Identification of Triticum asetivum-Haynaldia villosa Ditelosomic Addition Lines Involving Chromosome 2V of H.villosa
Abstract:Haynaldia villosa possesses a lots of elite traits,such as resistance to several diseases, tolerance to drought and cold, and is an important gene resource for wheat improvement.Triticum aestivum-Haynaldia villosa ditelosomic addition lines involving 2VL and 2VS were identified from the progenies of Triticum aestivum-Haynaldia villosa disomic substitution line 2V(2D) and Chinese Spring PhI (with Ph inhibit gene) by mitotic and meiotic analysis combining with chromosome C-banding and genomic in situ hybridization (GISH). The gene controlling bristles on the glume ridges was furtherly located on the short arm of 2V. Additionally, the SSR marker wmc25 was found to be tightly linked to the 2VS of H.villosa.
Keywords: Triticum aestivum Haynaldia villosa Ditelosomic addition lines Chromosome C-banding Genomic in situ hybridization SSR
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No.1691517640611975****
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