中国HIV/AIDS患者外周血单核细胞上TLR4表达和血浆TNF-α水平
首发时间:2007-12-20
摘要:目的 观察中国HIV/AIDS患者外周血单核细胞上Toll 样受体4(TLR4)表达水平和血浆TNF-α浓度, 初步探讨TLR4在HIV感染中的作用。方法 分别运用流式细胞术、ELISΑ法测定46例HIV/AIDS患者和30例健康对照者的外周血单核细胞上TLR4的表达及血浆TNF-α浓度, 采用t检验和相关分析进行数据分析。结果 HIV/AIDS患者外周血单核细胞TLR4的表达率、血浆TNF-α浓度分别是52.19±4.37%和35.79±5.08 pg/ml,均显著高于健康对照组;单核细胞上TLR4的表达率与血浆TNF-α浓度、HIV-1病毒载量的相关系数分别是0.645和0.708,呈正相关。结论 中国HIV/AIDS患者外周血单核细胞TLR4表达上调, 并且与患者体内HIV复制存在一定的相关性。
关键词: HIV/AIDS; Toll样受体4; 肿瘤坏死因子-α
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The expression of TLR4 on peripheral blood monocyte and TNF-α concentration of plasma in Chinese HIV/ΑIDS patients
Abstract:Objective Viewing the expression of TLR4 on peripheral blood monocyte of Chinese HIV/ΑIDS patients and the concentration of TNF-α in plasma. Method Using Flow Cytometry and ELISΑ method to detect the expression of TLR4 on peripheral blood monocyte and the concentration of TNF-α in plasma of 46 Chinese HIV/ΑIDS patients, comparing with 30 healthy control. Result The expression of TLR4 on monocyte and the concentration of TNF-α in plasma of Chinese HIV/ΑIDS patients are 52.19±4.37% and 35.79±5.08 pg/ml, which is higher than the healthy control significantly; The expression of TLR4 is positively correlated with the concentration of TNF-α and viral load in plasma, the coefficient is 0.645 and 0.708. Conclusion The expression of TLR4 on monocyte of Chinese HIV/ΑIDS patients is increased, which is associated with the replication of HIV.
Keywords: HIV/AIDS Toll-like receptor 4 Tumor necrosis factor-α
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No.1720617737211981****
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