水螨基因组DNA提取方法的研究
首发时间:2007-12-25
摘要:实验采用了CTAB、Chelex和PCR直接扩增法分别对寡毛刺触螨 Sperchon (Hispidosperchon)oligospinis、三叉腺水螨Lebertia(Pilolebertia)trifurcilla、阔毛曲跗湿螨Atractides latisetus、盐津急流水螨Torrenticola (Torrenticola) yanjinensis和紫色急流水螨Torrenticola (Monatractides) ianthina 5种不同的水螨进行了基因组DNA的提取,并通过PCR扩增COⅠ检测。结果显示CTAB法优于其它两种方法,活体和冷冻标本的提取效果优于液浸标本。
关键词: DNA提取 水螨 分子系统学
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The Study of the Methods of Genomic DNA Extracting from Water Mite
Abstract:Genomic DNA were extracted from 5 species of water mite, Sperchon (Hispidosperchon) oligospinis, Lebertia(Pilolebertia)trifurcilla, Atractides latisetus, Torrenticola (Torrenticola) yanjinensis, Torrenticola (Monatractides) ianthina , three different methods includes CTAB,Chelex and directly amplified with PCR were used. Then it was examined with the PCR of COⅠ. The result showed that the method of CTAB was better than the other two, the alived speciemans and the freezed speciemans were better than those were persented in liquid
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