洋葱细胞核仁结构与rDNA复制的动态关系
首发时间:2007-12-25
摘要:应用常规电子显微镜和电子显微镜放射自显影技术,对核仁超微结构在细胞分裂时期中的动态变化和rDNA的复制进行了研究。结果显示核仁内FC、DFC和GC结构在不同时期变化明显。核仁中rDNA的复制位置主要分布在核仁边缘的颗粒区和核仁相随染色质部位。rDNA的复制时期最早起始于G1期的较晚时期,此时只有少数细胞核仁中的rDNA进行复制;S期是rDNA复制的主要时期,复制比较活跃;在G2期复制并没有立即停止,rDNA的复制一直延续到G2期结束。
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The dynamic relationship between nucleolar structure
Abstract:By using the conventional electron microscopy and electron microscopic autoradiography, we investigated the morphological change of the nucleolar structure and the problem of rDNA replication in the cell cycle of Allium cepa. The results showed the nucleolar ultrastructure was high dynamic change in different stages of the cell cycle. The rDNA replication mostly took place in the granular component and nucleolar associated chromatin of the border region of nucleoli. In the late-G1 phase, rDNA replication began. S phase was the chief stage in replication, and the replication of rDNA changed more active. Then in G2 phase, the rDNA replication did not stop at once, and the rDNA replication lasted until G2 phase.
Keywords: Allium cepa nucleolus FC DFC rDNA replication
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