Effects of Acupuncture on Hepatic IRS-2mRNA Expression in Type 2 Diabetes Mellitus Model Rats

• 1、Second Clinical Medical College of Nanjing TCM

Abstract：Objective: To research the effect of rat’s hepatic IRS-2mRNA on pathologic mechanism of type 2 diabetes mellitus and the effect of acupuncture on the expression of hepatic IRS-2mRNA in T2DM model rats . Method: 50 of 100 SD male rats fed with high fat feedstuff became fat rats, which were injected in abdomen with Streptozotocin(25mg/kg) to make T2DM rat models. Then T2DM rats(18) were Stochastically devided into Glyburide group(6), acupuncture group(6) and model group(6). After treatment for 4 weeks, fast blood sugar(FBS) was examined by active blood sugar meter and fast insulin(FINS) was examined by radioimmunoassav(RIA) and expression of hepatic IRS-2mRNA was examined by real-time RT PCR, the results were compared with normal rats. Result: After treatment, the level of FBS in acupuncture and Glyburide group are obviously lower than model group(P<0.05); the level of FINS in acupuncture is markedly lower than model group(P<0.05), the level of FINS in Glyburide group is also lower than model group but there has no statistical meaning between them(P>0.05). The IRS-2mRNA of model group is 2.19-fold of normal group; which of Glyburide group is 4.59-fold of the normal group ; which of acupuncture group is 4.04% of the normal group. Conclusion: The abnormal expression of hepatic IRS-2mRNA may be one of the pathologic mechanism of T2DM. Acupuncture significantly depress the expression of hepatic IRS-2mRNA in T2DM rats.

Keywords： type 2 diabetes mellitus IRS-2mRNA real-time RT PCR acupuncture