RASSF1A基因在子宫颈癌组织中的表达
首发时间:2007-12-26
摘要:目的 探讨RASSF1A mRNA在子宫颈癌组织中的表达与子宫颈癌发生、发展的作用。方法 采用逆转录—聚合酶链反应(RT—PCR)方法检测56例子宫颈癌组织、36例子宫颈上皮内瘤样病变(CIN)组织和20例子宫颈正常组织中RASSF1A mRNA的表达。结果 正常宫颈、CIN和子宫颈癌组织中的RASSF1A mRNA的表达缺失率分别为0% (0 /20) 、22.2% (8 /36)和50.0% (28 /56) 。晚期( Ⅲ~Ⅳ期)宫颈癌组织中RASSF1A mRNA的表达缺失率(82.4% )明显高于其在早期( Ⅰ~Ⅱ期)宫颈癌组织中的表达缺失率(35.9% ) 。子宫颈鳞癌组织中RASSF1A mRNA的表达缺失率(63.3% )明显高于其在子宫颈腺癌中的表达缺失率(34.6% ) ( P < 0.05) 。子宫颈癌组织低分化组的RASSF1A mRNA的表达缺失率(70.8% )明显高于其在中、高分化组(37.5% )中的表达缺失率。结论 RASSF1A基因的表达缺失可能在子宫颈癌的发生、发展过程起着重要的作用。
关键词: RASSF1A基因;宫颈肿瘤
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Expression of RASSF1A mRNA in cervica cancer
Abstract:Objectives To study the expression of RASSF1A mRNA in cervical cancer. Methods Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of RASSF1A mRNA in 56 patients with cervical cancer, 36 patients with CIN and 20 patientswith normal cervix. Results The rates of loss of RASSF1A mRNA expression were 0% (0 /20) , 22. 2% ( 8 /36) and 50. 0% (28 /56) in patientswith normal cervix , CIN and cervical cancer respectively . The loss rate of RASSF1A mRNA in the cervical tissues at the advanced stages ( stages Ⅲ~Ⅳ, 82. 4% ) was higher than that at the early stages ( stages Ⅰ~Ⅱ, 35. 9% , P < 0. 05). RASSF1A mRNA deletion occurred more often in the tissues of squamous cervical cancers(63. 3% ) than in those of adenocarcinoma (34. 6% ) ( P < 0. 05). The loss rate of the poor differentiation group was 70. 8% , significantly higher than that of the middle and high differentiation group ( 37. 5% , P < 0. 05 ). Conclusion Loss of RASSF1A may play an important role in tumorgenesis and development of cervical cancer.
Keywords: RASSF1A genes Cervix neoplasms
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No.1737717885111986****
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