生长素、乙烯和一氧化氮相互作用对拟南芥下胚轴不定根形成的调控
首发时间:2007-12-27
摘要:本论文以拟南芥下胚轴插条作为实验系统,探讨生长素、乙烯和一氧化氮(NO)三者对拟南芥不定根形成的调节作用,以及生长素和乙烯信号传导成员在IAA促进不定根形成中的作用。结果表明:适宜浓度的IAA、ACC(乙烯合成前体)和SNP(NO供体)单独处理拟南芥切条7d均能促进不定根的形成,50μmol/L的IAA、0.1μmol/L的ACC和1μmol/L的SNP的促进不定根形成的作用最强。生长素、乙烯和NO对促进拟南芥形成不定根起协同作用;IAA和SNP的组合对不定根的促进作用最强。生长素和乙烯信号传导成员参与IAA促进不定根形成,特别是IAA14功能增强突变明显导致不定根形成数目减少,说明IAA14是IAA促进生根的正调节因子;而ARF17过量表达效果不明显。AUX1、AXR1、TIR1、ETR1、CTR1、EIN2和EIN3突变导致拟南芥下胚轴对IAA促进不定根形成作用敏感性降低。
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Interactions between auxin, ethylene and nitric oxide in adventitious rooting in Arabidopsis hypocotyl cuttings
Abstract:We employed arabidopsis hypocotyls cutting as experimental material to explore the effects of interactions between auxin, ethylene and nitric oxide on adventitious root formation, as well as the role of auxin and ethylene signaling components in the stimulatory effect of auxin on adventitious rooting. Results showed that the optimal concentration of IAA, ACC and SNP to rooting is 50μmol/L,0.1μmol/L and 1μmol/L. ACC and SNP play synergistic role with auin in adventitious root formation. Combination of auxin and SNP promotes more adventitious root formation than other treatment. Gain of function mutation of IAA14(SLR1) mutes the promotive effect of auxin on rooting, overexpression of ARF17 play little role in IAA’s effect on rooting, implying that IAA14 is the negative player in auxin signaling in adventitious rooting process. Mutations of AUX1, AXR1,TIR1,ETR1,CTR1,EIN2 and EIN3 result in decreased sensitivity of Arabidopsis hypocotyls to stimulatory effect of IAA on rooting.
Keywords: Auxin Ethylene Nitric oxide Adventitious root Hypocotyl cutting
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No.1744417935411987****
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