A novel molecular marker for the detection of Dasypyrum chromatin in wheat background

• 1、School of Life Science and Technology, University of Electronic Science and Technology of China

Abstract：In order to develop the molecular marker universally applied to the detection of the chromatin from Dasypyrum, which consists of annual Dasypyrum villosum and perennial D. breviaristatum. Studies showed that a Sabrina-like Long Terminal Repeat (LTR) retrotransposon sequence OPH121182 from D. breviaristatum genome could be used as molecular marker for detecting of D. breviaristatum and its derivatives. The aim of this study is to corroborate the feasibility of detecting the D. villosum chromatin and its derivatives using this marker. D. villosum accessions and wheat varieties or lines newly developed in Sichuan Province were tested using primer pair H12F and H12R, which was designed according to the sequence of OPH121182, the result showed that OPH121182 amplified in D. villosum accessions, Triticum- D. villosum amphiploid, 6V (6D) substitution line, 6VS/6AL translocation line, Shumai-375 and other several wheat lines, indicating that the marker could also be used for detection of D. villosum chromatin in wheat background. Furthermore, we also use the fluorescence in situ hybridization (FISH) of OPH121182 to identify the wheat - D. breviaristatum introgressions. It is concluded that OPH121182 was an effective molecular marker which can be widely used in chromosomal manipulation of wheat breeding using Dasypyrum species.

Keywords： Dasypyrum, Molecular marker In situ hybridization