超嗜热古菌Thermococcus sp.HJ21产高温α-葡萄糖苷酶
首发时间:2008-04-09
摘要:对一株分离自深海热液口的超嗜热古菌(Thermococcus sp. HJ21)菌株进行了产α-葡萄糖苷酶条件的优化和酶学性质的初步研究。结果表明:该菌株发酵时间为6h时,较适合产胞内α-葡萄糖苷酶,发酵时间为21h时较适合产胞外α-葡萄糖苷酶。其最适产酶温度为85℃,pH为6.5,NaCl浓度为2.5%。可溶性淀粉、酵母粉和蛋白胨促进产酶。该酶的最适作用温度为100℃,90℃半衰期(t1/2)为2h。最适酶作用pH值为7.0,在pH值5.0~8.0之间酶活力相对稳定。金属离子Cu2+、Al3+、Ni2+对该酶有较明显抑制作用,Hg2+几乎完全抑制该酶的活性,而EDTA、Fe3+和K+对该酶有激活作用。
关键词: 嗜热古菌 Thermococcus α-葡萄糖苷酶 酶学性质
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Production and Characterization of α-glucosidase from
Abstract:Abstract: The production and characterization of α-glucosidase from Thermococcus sp. HJ21 have been studied. Maximum production time of intracellular α-glucosidase and extracellular one were around 6h and 27h respectively. The temperature,pH and NaCl concentration of α-glucosidase production were 85℃, 6.5 and 2.5% respectively. Addition of soluble starch, yeast extract and peptone enhanced enzyme production. The optimal temperature of the α-glucosidase was at 100℃, and this enzyme exhibited extreme thermostability, with a half-life of 2h at 90℃.The α-glucosidase exhibited optimal activity at pH 7.0, and was stable at pH 5.0~8.0. The enzyme was more significantly inhibited by Cu2+,Al3+and Ni2+. Hg2+completely inactivateed the Thermococcus sp.HJ21 α-glucosidase. EDTA、 Fe3+and K+-treatments increased this enzyme activity.
Keywords: Hyperthermophilic Archaeon;Thermococcus;α-Glucosidase;Characterization
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No.2027121784712077****
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超嗜热古菌Thermococcus sp.HJ21产高温α-葡萄糖苷酶
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