肝癌细胞和正常肝细胞自体荧光光谱比较研究
首发时间:2008-06-16
摘要:本文采用荧光光谱技术对体外培养的SMMC-7721肝癌细胞和HL-7702正常肝细胞的自体荧光进行了研究。结果表明:两种细胞的自体荧光的发射光谱在波长为550-720nm范围内发射峰的数目和峰位均相同(602nm和691nm),只是主峰的波形略有差异。而在波长为720nm-800nm范围内,肝癌细胞有一特征荧光峰,峰位置在波长为734nm附近,而正常肝细胞则无此峰。通过对多代培养的细胞进行荧光光谱测试,发现两种细胞的荧光光谱特征在细胞传代的过程中保持不变,说明细胞中的组成物质在细胞传代过程中保持稳定。这两方面的实验为利用荧光光纤传感器来检测癌症打下了坚实的基础。
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The Study of Autofluorescence Spectrum between Hepatic Carcinoma Cell and Hepatic Cell
Abstract:The autofuorescence spectrum of and hepatic cell cultured in vitro was studied. It presented that the spectrogram of the hepatic carcinoma cell was resemble as that of the hepatic cell at the range of wavelength from 550nm to 720nm characterized by two peaks at 602nm and 691nm. But From 721nm to 800nm, the spectra of carcinoma cell with a peak located at 734nm were found. For the hepatic cell the peak at 734nm was not observed. The experimentations of propagation of the both cells confirm ulteriorly that there is a significant difference between two cells’ spectrum .
Keywords: Hepatic Carcinoma Cell Hepatic Cell Autofluorescence Spectrum
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No.2222926225512135****
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