Review on RAPD–PCR as a Discriminative Tool for Fish Bacteria

Lee Seong Wei; Najiah Musa; Wendy Wee; Chuah Tse Seng; Noor Azhar Mohd Shazili; Faizah Shaharom

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Review on RAPD – PCR as a Discriminative Tool for Fish Bacteria

首发时间：2008-06-16

Lee Seong Wei ¹ Najiah Musa ² Wendy Wee ² Chuah Tse Seng ³ Noor Azhar Mohd Shazili ⁴ Faizah Shaharom ⁵

1、Universiti Malaysia Terengganu
2、Department of Fisheries and Aquaculture
3、Department of Agrotechnology
4、Institute of Oceanography
5、Institute of Tropical Aquaculture (AQUATROP)Universiti

Abstract：This paper described application of random amplified polymorphic DNA- polymerase chain reaction (RAPD-PCR) to discriminate bacteria isolated from aquaculture sites. A total of 20 studies were reviewed in terms of types of primer used and its effectiveness as fish bacteria discrimination tool. RAPD-PCR was found to be a simple, cheap and rapid tool to discriminate fish bacteria especially at intraspecific level.

keywords： RAPD-PCR discriminate tool fish bacteria review

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Review on RAPD–PCR as a Discriminative Tool for Fish Bacteria

Lee Seong Wei ¹ Najiah Musa ² Wendy Wee ² Chuah Tse Seng ³ Noor Azhar Mohd Shazili ⁴ Faizah Shaharom ⁵

1、Universiti Malaysia Terengganu
2、Department of Fisheries and Aquaculture
3、Department of Agrotechnology
4、Institute of Oceanography
5、Institute of Tropical Aquaculture (AQUATROP)Universiti

摘要：This paper described application of random amplified polymorphic DNA- polymerase chain reaction (RAPD-PCR) to discriminate bacteria isolated from aquaculture sites. A total of 20 studies were reviewed in terms of types of primer used and its effectiveness as fish bacteria discrimination tool. RAPD-PCR was found to be a simple, cheap and rapid tool to discriminate fish bacteria especially at intraspecific level.

关键词： RAPD-PCR; discriminate tool; fish bacteria; review

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Lee Seong Wei,Najiah Musa,Wendy Wee ,et al. Review on RAPD – PCR as a Discriminative Tool for Fish Bacteria[EB/OL]. Beijing:Sciencepaper Online[2008-06-16]. http://www.paper.edu.cn/releasepaper/content/200806-333.

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论文编号	200806-333
论文题目	Review on RAPD–PCR as a Discriminative Tool for Fish Bacteria
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