野生欧洲李组织培养研究初报
首发时间:2008-10-02
摘要:本文以濒危果树野生欧洲李为外植体,进行组织培养研究,以期筛选出最优培养基配方及培养程序。结果表明:野生欧洲李初代培养时B5为基本培养基优于MS培养基。培养基中添加一定量激素的外植体生长势明显优于不添加激素的。以B5为基本培养基,6-BA浓度0.5-1.0 mg/L,并添加一定量的生长素(IBA、NAA)效果较优,能较快启动生长反应,并初步形成一定的增殖率。芽增殖培养阶段,6-BA浓度为0.5-1.0 mg/L时,以NAA为生长素时,适宜的激素比为NAA:6-BA=1:1-3;以IBA为生长素,IBA:6-BA=1:3-5较适宜。
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Primary Report on Tissue Culture of Prunus domestica L.
Abstract:The tissue culture of wild European plum (Prunus domestica L.)which is in severe danger was studied in this paper. The results showed that: In primary culture, B5 basal medium is better than MS. When add definite hormone in medium, explants had good growth than the medium without hormone. B5+ 6-BA 0.5~1.0 mg/L+NAA or IBA is most suitable for the primary culture. The suitable ratio for proliferation of buds is NAA:6-BA=1:1-3 or NAA:6-BA=1:3-5, with 6-BA 0.5~1.0 mg/L.
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No.2451631979612228****
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