Bt蛋白在不同矿物上的吸附动力学及其影响因素研究

刘洁; 胡红青; 李慧姝; 付庆灵; 黄丽

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Bt蛋白在不同矿物上的吸附动力学及其影响因素研究

首发时间：2008-10-17

刘洁 ¹ 胡红青 ¹ 李慧姝 ¹ 付庆灵 ¹ 黄丽 ¹

1、华中农业大学农业部亚热带农业资源与环境重点实验室

摘要：转Bt植物通过根系分泌等方式向土壤释放的毒素蛋白能吸附在土壤颗粒表面并累积，对土壤生态环境形成潜在风险，而目前缺乏针对Bt毒蛋白在土壤 (矿物) 表面吸附动力学的相关研究。本实验研究了Bt毒蛋白在针铁矿、二氧化硅、蒙脱石、高岭石四种矿物上的吸附动力学特征，并探讨了温度和pH的影响。结果表明Bt 毒蛋白能快速吸附在供试矿物表面，0.25h的吸附量约占平衡吸附量的75%-80%。其吸附动力学符合Elovich、二级扩散方程及抛物线扩散方程，但二级动力学方程的拟合程度更好。Bt蛋白在不同矿物表面的吸附量和二级吸附速率常数不同，且受温度和pH的影响。针铁矿在15℃-35℃内的吸附量随温度升高而下降，而蒙脱石、高岭石上变化趋势与针铁矿的相反，二氧化硅先下降后增加。Bt毒素在针铁矿和二氧化硅上吸附的二级速率常数 (K2) 随温度升高而明显降低，而蒙脱石和高岭石的K2先显著降低后略有升高。在实验pH范围内，Bt毒素在其等电点附近（pH 7）时的平衡吸附量最大，随pH增加其吸附量降低；而吸附速率常数则在供试矿物 (除二氧化硅外，其等电点不在实验pH范围内) 的等电点处出现。显然，Bt蛋白在矿物表面的吸附与蛋白和供试矿物本身的电化学性质、温度、pH等有关。

关键词： Bt蛋白土壤矿物吸附动力学影响因素

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Kinetic studies and affecting factors on adsorption of the toxin of Bacillus thuringiensis by minerals

Liu Jie ¹ Hu Hongqing ¹ Li Huishu ¹ Fu Qingling ¹ Huang Li ¹

1、School of Computer and Information Science, Hefei University of Technology

Abstract：The Bt toxin released by transgenic Bt plants could rapidly adsorbed and accumulated onto soil particles, to form potential risks on soil environment. To date, studies on the adsorption kinetic of the Bt toxin by minerals are rare, therefore the adsorption kinetic and affecting factors, such as temperature and pH, of the Bt toxin adsorbed by goethite, silicon dioxide, montmorillonite, and kaolinite was investigated at present study. The results indicated that Bt toxin could be rapidly adsorbed by minerals tested and the adsorption amount in the first 0.25 h was the 75%-80% of the equilibrium adsorption amount. The Elovich, pseudo-second-order, and parabolic diffusion equation were models to describe the adsorption kinetic, and the pseudo-second-order (PSO) model was the best one. The adsorption capability (the equilibrium adsorption amount qe and the rate constant K2 calculated by PSO model) of the minerals were different, and influenced by the temperature and pH. The qe of goethite decreased as the temperature varying from 15℃-35℃, while the qe of montmorillonite and kaolinite had the opposite performance. The PSO rate constant (K2) of the Bt toxin adsorbed by goethite and silicon dioxide were obviously decreased as the temperature increase, and K2 of montmorillonite and kaolinite were remarkable decreased firstly and then slightly increased. The equilibrium adsorption amount was highest at pH 7 which is the nearest point of the isoelectric point (IEP) of Bt toxin and decreased as the pH value increased to 9, and the PSO rate constant of the minerals (except silicon dioxide, its point of zero charge (PZC) is not in the pH range tested) were appeared at their point of zero charge, these results suggested that the adsorption of Bt toxin by minerals were related to the IEP of the toxin and the PZC of minerals.

Keywords： Bt toxin soil minerals kinetic of the adsorption effects on adsorption

基金：

1. 华中农业大学大学生科技创新基金,教育部高校博士点基金,国家自然科学基金（A07103,20050504011, 40671087）

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刘洁，胡红青，李慧姝，等. Bt蛋白在不同矿物上的吸附动力学及其影响因素研究[EB/OL]. 北京：中国科技论文在线 [2008-10-17]. https://www.paper.edu.cn/releasepaper/content/200810-442.

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论文编号	200810-442
论文题目	Bt蛋白在不同矿物上的吸附动力学及其影响因素研究
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