Study on Polymorphism of Artemisia capillaris By Using RAPD in Terengganu, Malaysia.
首发时间:2008-11-07
Abstract:Artemisia capillaris is a herbaceous aromatic and therapeutic plant. The genetic variability among individuals of Artemisia capillaris from state of Terengganu, Malaysia was examined by using the random amplified polymorphic DNA (RAPD) technique to assess the polymorphism at the species level. The samples from differences regional in Terengganu State. The genomic DNA was extracted from the samples leaves using Sarkosyl method. The results produced by the machine showed clear RAPD banding pattern. Fifthty-seven oligonucleotide primers were screened and five primers were selected (OPA 04, OPA 09, OPA 16, OPA 17 and OPA 18) to amplify DNA from five samples of Artemisia capillaris from State of Terengganu, Malaysia. A total of 135 RAPD fragments (RAPDs) with all polymorphic fragments (100%) with size ranging from 250 – 3000 bp were scored from the population. Genetic distance for samples ranges from 0.0000 to 0.320000. For similarity index samples ranges from 0.0000 to 0.7547.
keywords: Artemisia capillaris Polymorphism Genetic variability RAPD
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Study on Polymorphism of Artemisia capillaris By Using RAPD in Terengganu, Malaysia.
摘要:Artemisia capillaris is a herbaceous aromatic and therapeutic plant. The genetic variability among individuals of Artemisia capillaris from state of Terengganu, Malaysia was examined by using the random amplified polymorphic DNA (RAPD) technique to assess the polymorphism at the species level. The samples from differences regional in Terengganu State. The genomic DNA was extracted from the samples leaves using Sarkosyl method. The results produced by the machine showed clear RAPD banding pattern. Fifthty-seven oligonucleotide primers were screened and five primers were selected (OPA 04, OPA 09, OPA 16, OPA 17 and OPA 18) to amplify DNA from five samples of Artemisia capillaris from State of Terengganu, Malaysia. A total of 135 RAPD fragments (RAPDs) with all polymorphic fragments (100%) with size ranging from 250 – 3000 bp were scored from the population. Genetic distance for samples ranges from 0.0000 to 0.320000. For similarity index samples ranges from 0.0000 to 0.7547.
关键词: Artemisia capillaris Polymorphism Genetic variability RAPD
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