细胞色素C 和ATP对离体梨花粉管生长及其核DNA稳定性的影响
首发时间:2008-12-31
摘要:为探讨梨花粉管衰老过程中是否存在程序性死亡的信号路径,分别用外源细胞色素c和ATP对梨花粉(管)进行单独或混合处理,检测细胞色素c能否使离体花粉管发生程序性死亡。结果表明,细胞色素c和ATP混合处理的四个组合都能抑制离体花粉萌发和花粉管生长,并且引起了花粉管核DNA的降解,不同处理中双核、单核及无核花粉管所占比率存在明显差异;高浓度ATP虽能抑制花粉萌发和花粉管生长,但不能引起花粉管核DNA的降解。本研究首次发现了梨花粉管停止生长过程具有PCD过程的典型特征,花粉管停长的死亡过程是属于PCD类型,为研究自交不亲和性反应中自花花粉管停止生长的机制提供新的思路。
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The Effect of Cytochrome c and ATP on the Pollen Germination and the Degradation of Nuclear DNA
Abstract:To investigate whether programmed cell death (PCD) can be triggered during the decrepit course in the pollen tubes of pear, the exogenous cytochrome c and ATP were respectively or together incubated with pollen grains and pollen tubes. The results showed that the four treatments of cytochrome c supplemented with ATP all suppressed the pollen germination and pollen tube growth, and induced nuclear DNA degradation, different treatments with different ratios of double nuclei, single nucleus, and no nuclei. Treatment with heavy concentration of ATP also restrained pollen germination and pollen tube growth, but can not induced nuclear DNA degration. It was the first time to make sure that the diagnostic characters of PCD was observed in the growth inhibiting pollen tubes of pear, which indicated the death of pollen tube belong to the PCD, and provided new eyeshot on the study of pollen tube death in the self-incompatibility.
Keywords: pear cytochrome c pollen tube Nuclear DNA degradation programmed cell death
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