从活性污泥中培养驯化分离苯酚降解菌的实验研究
首发时间:2008-12-24
摘要:苯酚是造纸、炼焦、炼油、塑料、农药、医药合成等行业生产的原料或中间体。随着经济的发展,未经处理的含酚废水对人类的生存环境已经造成了严重的威胁。利用微生物降解的方法处理含酚废水是一种经济有效且无二次污染的方法。在生物处理过程中,由于含酚废水的难降解特性,因此引入的菌种要有较强的降解苯酚能力,能较好地适应冲击负荷的变化。本文研究的是含苯酚废水的生物处理,即以培养高效菌株为主要研究内容,从吉林省长春市第一汽车厂的污水处理站的污水、污泥中筛选到几株高效的苯酚降解细菌,能够在以苯酚为唯一碳源和能源的培养基上生长。该菌株菌落呈白色,表面光滑。研究采用逐渐提高苯酚浓度的方法驯化苯酚降解菌,使其对苯酚的降解能力从300mg/L提高到1000mg/L 。从对驯化培养的菌株对苯酚降解率探讨可知,该菌株的生长和对苯酚的降解是基本同步的,而且由于各菌之间存在协同降解作用,混合菌株对苯酚的降解在各方面都优于纯菌种。
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The experiment studying of cultivating,domesticating and separating the phenol degrading bacterial from activated sludge
Abstract:Phenol is an important raw material or midst substance of production in paper making, coking, oil refining, plastic, pesticide and medicine synthesis industries. With the development of economy, the phenol wastewater without being treated has been imposed a serious threat on our environment. It is an economic and effective way to treat phenol wastewater with the method of microorganism biodegradation. Moreover, the way does not have second pollution. Since phenol wastewater has a trait in difficult degradation, bacterium is introduced into the process of organism treatment to degrade phenol and adapt to the changes of impingement load. Based on the above problem, biodegradation of phenol wastewater is studied and main study object is to cultivate well-performed strains, which can grow on the cultivating group based on phenol as the sole source of carbon and energy, and these strains were filtered from wastewater and soil samples which were polluted by phenol wastewater at the wastewater treatment station of the First Car Factory in Changchun, Jin Lin province. The color of the strain is white, which surface is smooth. The method is to gradually raise the condensation degree of phenol for bacterium step by step here. The result shows that the phenol degrading abilities are greatly promoted from 300mg/L to 1000mg/L. Through studying its degrading rate, it is found that the growth and phenol degradation of bacterium are nearly synchronic. In addition, due to the co-degradation effect among bacteria, the mixture bacterial strains have a more advantaged performance on degrading phenol than what pure bacterial strains do in this experiment.
Keywords: phenol wastewater biodegradation domestication phenol degrading bacterial
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No.2690636883912301****
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